| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IHC-P:1:50-1:200 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | HDAC8 |
| Gene ID: | 55869 |
| Uniprot ID: | HDAC8_HUMAN |
| Immunogen Region: | 1-377 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-377 of human HDAC8 (NP_060956.1). |
| Immunogen Sequence: | MEEPEEPADSGQSLVPVYIY SPEYVSMCDSLAKIPKRASM VHSLIEAYALHKQMRIVKPK VASMEEMATFHTDAYLQHLQ KVSQEGDDDHPDSIEYGLGY DCPATEGIFDYAAAIGGATI TAAQCLIDGMCKVAINWSGG WHHAKKDEASGFCYLNDAVL GILRLRRKFERILYVDLDLH HGDGVEDAFSFTSKVMTVSL HKFSPGFFPGTGDVSDVGLG KGRYYSVNVPIQDGIQDEK |
| Tissue Specificity | Weakly expressed in most tissues. Expressed at higher level in heart, brain, kidney and pancreas and also in liver, lung, placenta, prostate and kidney. |
| Post Translational Modifications | Phosphorylated by PKA on serine 39. Phosphorylation reduces deacetylase activity observed preferentially on histones H3 and H4. |
| Function | Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Also involved in the deacetylation of cohesin complex protein SMC3 regulating release of cohesin complexes from chromatin. May play a role in smooth muscle cell contractility. In addition to protein deacetylase activity, also has protein-lysine deacylase activity: acts as a protein decrotonylase by mediating decrotonylation ((2E)-butenoyl) of histones. |
| Protein Name | Histone Deacetylase 8 Hd8 Protein Deacetylase Hdac8 Protein Decrotonylase Hdac8 |
| Database Links | Reactome: R-HSA-2122947 Reactome: R-HSA-2467813 Q9BY41-1 Reactome: R-HSA-2500257 Q9BY41-1 Reactome: R-HSA-2644606 Reactome: R-HSA-2894862 Reactome: R-HSA-3214815 Reactome: R-HSA-350054 |
| Cellular Localisation | Nucleus Chromosome Cytoplasm Excluded From The Nucleoli Found In The Cytoplasm Of Cells Showing Smooth Muscle Differentiation |
| Alternative Antibody Names | Anti-Histone Deacetylase 8 antibody Anti-Hd8 antibody Anti-Protein Deacetylase Hdac8 antibody Anti-Protein Decrotonylase Hdac8 antibody Anti-HDAC8 antibody Anti-HDACL1 antibody Anti-CDA07 antibody |
Information sourced from Uniprot.org