• STJ73163 (0. 1µg/ml) staining of Jurkat nuclear lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-HDAC3 antibody (Internal) (STJ73163)

SKU:
STJ73163-100

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Host: Goat
Applications: Pep-ELISA/WB
Reactivity: Human/Mouse/Rat/Dog/Pig/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-HDAC3 (Internal) is suitable for use in ELISA and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.1-0.3µg/ml
ELISA-antibody detection limit dilution 1:128000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: HDAC3
Gene ID: 8841
Uniprot ID: HDAC3_HUMAN
Immunogen Region: Internal
Accession Number: NP_003874.2
Immunogen Sequence: NHAPSVQIHDVPAD
Post Translational Modifications Sumoylated in vitro. Deubiquitinated on 'Lys-63'-linked ubiquitin chains by USP38.leading to a decreased level of histone acetylation.
Function Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates. Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Participates in the BCL6 transcriptional repressor activity by deacetylating the H3 'Lys-27' (H3K27) on enhancer elements, antagonizing EP300 acetyltransferase activity and repressing proximal gene expression. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation. Contributes, together with XBP1 isoform 1, to the activation of NFE2L2-mediated HMOX1 transcription factor gene expression in a PI(3)K/mTORC2/Akt-dependent signaling pathway leading to endothelial cell (EC) survival under disturbed flow/oxidative stress. Regulates both the transcriptional activation and repression phases of the circadian clock in a deacetylase activity-independent manner. During the activation phase, promotes the accumulation of ubiquitinated BMAL1 at the E-boxes and during the repression phase, blocks FBXL3-mediated CRY1/2 ubiquitination and promotes the interaction of CRY1 and BMAL1. The NCOR1-HDAC3 complex regulates the circadian expression of the core clock gene BMAL1 and the genes involved in lipid metabolism in the liver. Also functions as deacetylase for non-histone targets, such as KAT5, MEF2D, MAPK14 and RARA. Serves as a corepressor of RARA, mediating its deacetylation and repression, leading to inhibition of RARE DNA element binding. In association with RARA, plays a role in the repression of microRNA-10a and thereby in the inflammatory response. In addition to protein deacetylase activity, also acts as protein-lysine deacylase by recognizing other acyl groups: catalyzes removal of (2E)-butenoyl (crotonyl) and 2-hydroxyisobutanoyl (2-hydroxyisobutyryl) acyl groups from lysine residues, leading to protein decrotonylation and de-2-hydroxyisobutyrylation, respectively. Catalyzes decrotonylation of MAPRE1/EB1.
Protein Name Histone Deacetylase 3
Hd3
Protein Deacetylase Hdac3
Protein Deacylase Hdac3
Rpd3-2
Smap45
Database Links Reactome: R-HSA-1368071
Reactome: R-HSA-193670
Reactome: R-HSA-1989781
Reactome: R-HSA-2122947
Reactome: R-HSA-2151201
Reactome: R-HSA-2644606
Reactome: R-HSA-2894862
Reactome: R-HSA-3214815
Reactome: R-HSA-350054
Reactome: R-HSA-381340
Reactome: R-HSA-390471
Reactome: R-HSA-400206
Reactome: R-HSA-400253
Reactome: R-HSA-5617472
Reactome: R-HSA-8940973
Reactome: R-HSA-8943724
Reactome: R-HSA-9022537
Reactome: R-HSA-9022692
Reactome: R-HSA-9029569
Reactome: R-HSA-9609690
Reactome: R-HSA-9701898
Reactome: R-HSA-9707564
Reactome: R-HSA-9707616
Cellular Localisation Nucleus
Cytoplasm
Cytosol
Colocalizes With Xbp1 And Akt1 In The Cytoplasm
Predominantly Expressed In The Nucleus In The Presence Of Ccar2
Alternative Antibody Names Anti-Histone Deacetylase 3 antibody
Anti-Hd3 antibody
Anti-Protein Deacetylase Hdac3 antibody
Anti-Protein Deacylase Hdac3 antibody
Anti-Rpd3-2 antibody
Anti-Smap45 antibody
Anti-HDAC3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance