![Anti-Part-Humanised-HCV subtype 1b NS5B protein antibody (111-130 aa) [9A2] (STJA0002829)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/150095/299807/STJA0002829_1__64148.1633363903.jpg?c=1 "Anti-Part-Humanised-HCV subtype 1b NS5B protein antibody (111-130 aa) [9A2] (STJA0002829)")
![Anti-Part-Humanised-HCV subtype 1b NS5B protein antibody (111-130 aa) [9A2] (STJA0002829)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/150095/299807/STJA0002829_1__64148.1633363903.jpg?c=1 "Anti-Part-Humanised-HCV subtype 1b NS5B protein antibody (111-130 aa) [9A2] (STJA0002829)")
Anti-Part-Humanised-HCV subtype 1b NS5B protein antibody (111-130 aa) [9A2] (STJA0002829)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
ImmunogenRecombinant Hepatitis C Virus subtype 1b nonstructural protein 5B (NS5B) RNA-dependent RNA polymerase (RdRp). Mapped to amino acids 111-130 (LSSKAVNHIRSVWKDLLEDT)
General Information
| Short Description | Mouse monoclonal anti-Part-Humanised-HCV subtype 1b NS5B protein (111-130 aa) for use in ELISA, WB and IF in Human and HCV samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ELISA/WB/IF |
| Host | Mouse |
| Reactivity | Human/HCV |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 9A2 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Purification | NA Protein G purification NA |
| Dilution Range | ELISA titer 11000; Western immunoblotting, reduced conditions; dilution 11000; Immunofluorescense 11000 Monoclonal antibody working titer has to be established practically for each particular antigen and assay format. |
| Formulation | PBS pH 7.4, with 0.1% sodium azide |
| Storage Instruction | Store at-20 to-70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result. |
Target Information
| Immunogen | Recombinant Hepatitis C Virus subtype 1b nonstructural protein 5B (NS5B) RNA-dependent RNA polymerase (RdRp). Mapped to amino acids 111-130 (LSSKAVNHIRSVWKDLLEDT) |
| Immunogen Region | 111-130 aa |
| Specificity | Human, HCV subtype 1b NS5B, Epitope mapped to amino acids 111-130 (LSSKAVNHIRSVWKDLLEDT) |
Additional Info
| Background | Non-structural protein 5B (NS5B) represents the RNA-dependent RNA polymerase (RdRp) of Hepatitis C Virus, which is a small positive strand RNA virus in the family Flaviviridae. HCV is a major causative agent of acute and chronic hepatitis, hepatocellular carcinoma and liver cirrhosis. The single subunit RNA-dependent RNA polymerase is absolutely essential for the viral replication. Antibody is derived from mouse anti-HCV NS5B monoclonal antoibody 9A2 expressing mouse hybridoma culture. cDNA of antibody variable regions were isolated and cloned into the human IgG1 constant region-containing antibody expression vector. Partially humanized, chimeric antibody was expressed by QMCF CHOEBNALT85 cell line. Antibody is used for high-specific efficient detection of soluble or membrane-bound proteins fused with 9A2 epitope-tag sequence. |
Information sourced from Uniprot.org