| Host: |
Mouse |
| Applications: |
ELISA/WB/IF |
| Reactivity: |
Human/HCV |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-Part-Humanised-HCV subtype 1b NS5B protein (111-130 aa) is suitable for use in ELISA, Western Blot and Immunofluorescence research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
9A2 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG1 |
| Formulation: |
PBS pH 7.4, with 0.1% sodium azide |
| Purification: |
NA Protein G purification NA |
| Dilution Range: |
ELISA titer 11000; Western immunoblotting, reduced conditions; dilution 11000; Immunofluorescense 11000 Monoclonal antibody working titer has to be established practically for each particular antigen and assay format. |
| Storage Instruction: |
Store at-20 to-70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result. |
| Immunogen Region: |
111-130 aa |
| Specificity: |
Human, HCV subtype 1b NS5B, Epitope mapped to amino acids 111-130 (LSSKAVNHIRSVWKDLLEDT) |
| Immunogen: |
Recombinant Hepatitis C Virus subtype 1b nonstructural protein 5B (NS5B) RNA-dependent RNA polymerase (RdRp). Mapped to amino acids 111-130 (LSSKAVNHIRSVWKDLLEDT) |
| Background | Non-structural protein 5B (NS5B) represents the RNA-dependent RNA polymerase (RdRp) of Hepatitis C Virus, which is a small positive strand RNA virus in the family Flaviviridae. HCV is a major causative agent of acute and chronic hepatitis, hepatocellular carcinoma and liver cirrhosis. The single subunit RNA-dependent RNA polymerase is absolutely essential for the viral replication. Antibody is derived from mouse anti-HCV NS5B monoclonal antoibody 9A2 expressing mouse hybridoma culture. cDNA of antibody variable regions were isolated and cloned into the human IgG1 constant region-containing antibody expression vector. Partially humanized, chimeric antibody was expressed by QMCF CHOEBNALT85 cell line. Antibody is used for high-specific efficient detection of soluble or membrane-bound proteins fused with 9A2 epitope-tag sequence. |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
