| Short Description : | Mouse monoclonal anti-HCV subtype 1b NS5B protein (95-105 aa) for use in ELISA, WB and IP in Human and HCV samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | ELISA/WB/IP |
| Host: | Mouse |
| Reactivity: | Human/HCV |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | 7G8 |
| Isotype: | NA IgG1 NA |
| Conjugation: | Unconjugated |
| Purification: | Protein G purification |
| Dilution Range: | WB in reduced conditions 1 µg/ml; Optimal conditions for IP should be determined for each particular application.Blocks the RNA-dependent RNA polymerase activity in vitro.Monoclonal antibody working titer has to be established practically for each pa |
| Formulation: | PBS pH 7.4, with 0.1% sodium azide |
| Storage Instruction: | Store at-20 to-70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result. |
| Immunogen: | Recombinant Hepatitis C Virus subtype 1b nonstructural protein 5B (NS5B). Mapped to amino acids 95-105 (TPPHSARSKFGYGA) |
| Immunogen Region: | 95-105 aa |
| Specificity: | Human, HCV subtype 1b NS5B, Epitope mapped to amino acids 95-105 (TPPHSARSKFGYGA) |
| Background | Non-structural protein 5B (NS5B) represents the RNA-dependent RNA polymerase (RdRp) of Hepatitis C Virus, which is a small positive strand RNA virus in the family Flaviviridae. HCV is a major causative agent of acute and chronic hepatitis, hepatocellular carcinoma and liver cirrhosis. The single subunit RNA-dependent RNA polymerase is absolutely essential for the viral replication. |
Information sourced from Uniprot.org