• Western blot analysis of lysates from Human coronavirus (HCoV-OC43) Spike Protein (S1+S2 ECDHis Tag) , using HCoV-OC43 Spike S2 Rabbit polyclonal antibody (STJ11103407) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-HCoV-OC43 Spike S2 antibody (782-881) (STJ11103407)

SKU:
STJ11103407

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Host: Rabbit
Applications: WB/ELISA
Reactivity: HCoV-OC43
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 782-881
Specificity: A synthetic peptide corresponding to a sequence within amino acids 782-881 of coronavirus Spike S2 (YP_009555241.1).
Immunogen Sequence: EPVGGLYEIQIPSEFTIGNM VEFIQTSSPKVTIDCAAFVC GDYAACKSQLVEYGSFCDNI NAILTEVNELLDTTQLQVAN SLMNGVTLSTKLKDGVNFNV
Background S1 attaches the virion to the cell membrane by interacting with sialic acid-containing cell receptors, initiating the infection.; [Spike protein S1]: attaches the virion to the cell membrane by interacting with host receptor, initiating the infection.

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance