• Western blot analysis of lysates from Human coronavirus (HCoV-OC43) Spike Protein (S1+S2 ECDHis Tag) , using HCoV-OC43 Spike S2 Rabbit polyclonal antibody (STJ11103407) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-HCoV-OC43 Spike S2 antibody (782-881) (STJ11103407)

SKU:
STJ11103407

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Host: Rabbit
Applications: WB
Reactivity: HCoV-OC43
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HCoV-OC43 Spike S2 (782-881) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 782-881
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 782-881 of coronavirus Spike S2 (YP_009555241.1).
Immunogen Sequence: EPVGGLYEIQIPSEFTIGNM VEFIQTSSPKVTIDCAAFVC GDYAACKSQLVEYGSFCDNI NAILTEVNELLDTTQLQVAN SLMNGVTLSTKLKDGVNFNV
Background The protein encoded by this gene was first identified by its ability to bind the adenovirus E1A protein. The protein localizes to the nucleus. It functions as a transcriptional repressor, and expression of E1A inhibits this repression. Alternatively spliced transcript variants encoding different isoforms have been identified.

Information sourced from Uniprot.org

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