Anti-HAS3 antibody (67-281) (STJ28700)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal HAS3 (67-281) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | HAS3 |
| Gene ID | 3038 |
| Uniprot ID | HYAS3_HUMAN |
| Immunogen Region | 67-281 |
| Immunogen Sequence | HRRMRRAGQALKLPSPRRGS VALCIAAYQEDPDYLRKCLR SAQRISFPDLKVVMVVDGNR QEDAYMLDIFHEVLGGTEQA GFFVWRSNFHEAGEGETEAS LQEGMDRVRDVVRASTFSCI MQKWGGKREVMYTAFKALGD SVDYIQVCDSDTVLDPACTI EMLRVLEEDPQVGGVGGDVQ PPGKGMAVEDDQVQAAQVRA TEAWSVHQRHVSREQ |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 67-281 of human HAS3 (NP_619515.1). |
Additional Info
| Post Translational Modifications | O-GlcNAcylation increases the hyaluronan synthase activity, HAS3 stability and its plasma membrane residence. The concentration of UDP-GlcNAc controls the level of O-GlcNAc modification. |
| Function | Catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer. Therefore, it is essential to hyaluronan synthesis a major component of most extracellular matrices that has a structural role in tissues architectures and regulates cell adhesion, migration and differentiation. This is one of three isoenzymes responsible for cellular hyaluronan synthesis. |
| Protein Name | Hyaluronan Synthase 3Hyaluronate Synthase 3Hyaluronic Acid Synthase 3Ha Synthase 3 |
| Database Links | Reactome: R-HSA-2142850 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinGolgi Apparatus MembraneGolgi ApparatusTrans-Golgi Network MembraneEarly EndosomeTravels From Endoplasmic Reticulum (Er)Golgi To Plasma MembraneActives Only When Present In Plasma MembraneO-Glcnacylation Controls Its Membrane LocalizationA Rapid Recycling Of Has3 Between Plasma Membrane And Endosomes Is Controlled By The Cytosolic Levels Of Udp-Glcua And Udp-Glcnac |
| Alternative Antibody Names | Anti-Hyaluronan Synthase 3 antibodyAnti-Hyaluronate Synthase 3 antibodyAnti-Hyaluronic Acid Synthase 3 antibodyAnti-Ha Synthase 3 antibodyAnti-HAS3 antibody |
Information sourced from Uniprot.org