• Western blot analysis of extracts of various cell lines, using HARS2 antibody (STJ110095) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-HARS2 antibody (217-506) (STJ110095)

SKU:
STJ110095

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HARS2 (217-506) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HARS2
Gene ID: 23438
Uniprot ID: SYHM_HUMAN
Immunogen Region: 217-506
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 217-506 of human HARS2 (NP_036340.1).
Immunogen Sequence: IVDGMFAVCGVPESKFRAIC SSIDKLDKMAWKDVRHEMVV KKGLAPEVADRIGDYVQCHG GVSLVEQMFQDPRLSQNKQA LEGLGDLKLLFEYLTLFGIA DKISFDLSLARGLDYYTGVI YEAVLLQTPTQAGEEPLNVG SVAAGGRYDGLVGMFDPKGH KVPCVGLSIGVERIFYIVEQ RMKTKGEKVRTTETQVFVAT PQKNFLQERLKLIAELWDSG IKAEMLYKNNPKLLTQLHY
Tissue Specificity A high level expression is seen in the heart, kidney and skeletal muscle while a lower level expression is seen in the brain and liver.
Function Mitochondrial aminoacyl-tRNA synthetase that catalyzes the ATP-dependent ligation of histidine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (His-AMP).
Protein Name Histidine--Trna Ligase - Mitochondrial
Histidine--Trna Ligase-Like
Histidyl-Trna Synthetase
Hisrs
Database Links Reactome: R-HSA-379726
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Histidine--Trna Ligase - Mitochondrial antibody
Anti-Histidine--Trna Ligase-Like antibody
Anti-Histidyl-Trna Synthetase antibody
Anti-Hisrs antibody
Anti-HARS2 antibody
Anti-HARSL antibody
Anti-HARSR antibody
Anti-HO3 antibody

Information sourced from Uniprot.org

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