• Immunofluorescence analysis of HepG2 and HeLa cells, using HARS Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of lysates from COLO cells, using HARS Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using HisRS Polyclonal Antibody diluted at 1:2000

Anti-HARS1 antibody (460-509 aa) (STJ93504)

SKU:
STJ93504

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Histidine--tRNA ligase, cytoplasmic (460-509 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HARS1
Gene ID: 3035
Uniprot ID: HARS1_HUMAN
Immunogen Region: 460-509 aa
Specificity: HisRS Polyclonal Antibody detects endogenous levels of HisRS protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human HARS at the amino acid range 460-509
Function Catalyzes the ATP-dependent ligation of histidine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (His-AMP). Plays a role in axon guidance.
Protein Name Histidine--Trna Ligase - Cytoplasmic
Histidyl-Trna Synthetase
Hisrs
Database Links Reactome: R-HSA-379716
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Histidine--Trna Ligase - Cytoplasmic antibody
Anti-Histidyl-Trna Synthetase antibody
Anti-Hisrs antibody
Anti-HARS1 antibody
Anti-HARS antibody
Anti-HRS antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance