| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-HABP4 (50-150) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
HABP4 |
| Gene ID: |
22927 |
| Uniprot ID: |
HABP4_HUMAN |
| Immunogen Region: |
50-150 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human HABP4 (NP_055097.2). |
| Immunogen Sequence: |
QQQLQRKRRDEAAAAAGAGP RGGRSPAGASGHRAGAGGRR ESQKERKSLPAPVAQRPDSP GGGLQAPGQKRTPRRGEQQG WNDSRGPEGMLERAERRSYR E |
| Tissue Specificity | Highly expressed in brain, heart, and kidney, and moderately expressed in skeletal muscle. Also expressed in a variety of tumor cell lines and in activated but not resting leukocytes. |
| Post Translational Modifications | Methylated. Methylation is decreased by phorbol 12-myristate 13-acetate (PMA)-activated PKC, in vitro. Phosphorylated by phorbol 12-myristate 13-acetate (PMA)-activated PKC isoforms at Thr-354 and Thr-375. |
| Function | Ribosome-binding protein that promotes ribosome hibernation, a process during which ribosomes are stabilized in an inactive state and preserved from proteasomal degradation. Acts via its association with EEF2/eEF2 factor at the A-site of the ribosome, promoting ribosome stabilization in an inactive state compatible with storage. Plays a key role in ribosome hibernation in the mature oocyte by promoting ribosome stabilization. Ribosomes, which are produced in large quantities during oogenesis, are stored and translationally repressed in the oocyte and early embryo. Also binds RNA, regulating transcription and pre-mRNA splicing. Binds (via C-terminus) to poly(U) RNA. Seems to play a role in PML-nuclear bodies formation. Negatively regulates DNA-binding activity of the transcription factor MEF2C in myocardial cells in response to mechanical stress. |
| Protein Name | Intracellular Hyaluronan-Binding Protein 4Ihabp-4Ihabp4Hyaluronan-Binding Protein 4Ki-1/57 Intracellular Antigen |
| Database Links | Reactome: R-HSA-114608 |
| Cellular Localisation | NucleusCytoplasmStress GranuleSarcoplasmNuclear BodyNucleolusNucleus SpeckleCajal BodyGemTransported Into The Nuclear Compartment In Activated LeukocytesInhibition Of Methylation Alters Its Distribution Between The Nuclear And Cytoplasmic CompartmentsMethylation May Be Required For Its Localization In Subnuclear StructuresSuch As NucleoliNuclear SpecklesCajal Bodies And Gemini Of Coiled Bodies (Gems)Colocalizes With Fmr1Fxr1 And Fxr2 In Cytoplasmic Stress GranulesIn Myocardial CellsLocalization At The Sarcoplasm Is Reduced In Response To Mechanical Stress |
| Alternative Antibody Names | Anti-Intracellular Hyaluronan-Binding Protein 4 antibodyAnti-Ihabp-4 antibodyAnti-Ihabp4 antibodyAnti-Hyaluronan-Binding Protein 4 antibodyAnti-Ki-1/57 Intracellular Antigen antibodyAnti-HABP4 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
