• Western blot analysis of extracts of 293T cells, using rabbit anti HA-Tag antibody (STJ11103705) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of 293T-HA-C and 293T-HA-N cells using Rabbit anti HA-Tag polyclonal antibody (STJ11103705) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of 293T-HA-C and 293T-HA-N cells using rabbit anti HA-Tag polyclonal antibody (STJ11103705) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from 293T cells, using Rabbit anti HA-Tag polyclonal antibody (STJ11103705) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug rabbit anti HA-Tag antibody (STJ11103705). Western blot was performed from the immunoprecipitate using rabbit anti HA-Tag antibody (STJ11103705) at a dilition of 1:2000.

Anti-HA-Tag antibody (STJ11103705)

SKU:
STJ11103705

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Species independent
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-HA-Tag is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:2000-1:6000
IF 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: A synthetic peptide corresponding to HA-Tag.
Immunogen Sequence: YPVDVPDYA
Background The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Three transcript variants encoding different isoforms have been found for this gene.

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance