• Western blot analysis of extracts of 293T cells, using mouse anti HA-Tag monoclonal antibody antibody (STJ11103706) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of extracts of 293T cells, using mouse anti HA-Tag monoclonal antibody antibody (STJ11103706) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of extracts of 293T cells, using mouse anti HA-Tag monoclonal antibody antibody (STJ11103706) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of 293T cells, using mouse anti HA-Tag monoclonal antibody antibody (STJ11103706) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug mouse anti HA-Tag antibody (STJ11103706). Western blot was performed from the immunoprecipitate using mouse anti HA-Tag antibody (STJ11103706) at a dilition of 1:1000.

Anti-HA-Tag antibody [S6MM] (STJ11103706)

SKU:
STJ11103706

£140.50 - £212.50
Current Stock:
Host: Mouse
Applications: WB/IP
Reactivity: Species independent
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-HA-Tag is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S6MM
Conjugation: Unconjugated
Isotype: IgG1k
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IP 1:1000-1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: A synthetic peptide corresponding to HA tag.
Immunogen Sequence: YPVDVPDYA
Background This gene is a member of the M-phase phosphoprotein (MPP) family. The gene encodes a phosphoprotein with a J domain and a Myb DNA-binding domain which localizes to both the nucleus and the cytosol. The protein is capable of forming a heterodimeric complex that associates with ribosomes, acting as a molecular chaperone for nascent polypeptide chains as they exit the ribosome. This protein was identified as a leukemia-associated antigen and expression of the gene is upregulated in leukemic blasts. Also, chromosomal aberrations involving this gene are associated with primary head and neck squamous cell tumors. This gene has a pseudogene on chromosome 6. Alternatively spliced variants which encode different protein isoforms have been described.

Information sourced from Uniprot.org

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