Anti-H6PD/GDH antibody (541-791 aa) [SRM5393] (STJ11107303)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal H6PD/GDH (541-791 aa) antibody for use in IF, ICC and ELISA in mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SRM5393 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | IF/ICC:1:100-1:400ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | H6PD |
| Gene ID | 9563 |
| Uniprot ID | G6PE_HUMAN |
| Immunogen Region | 541-791 aa |
| Immunogen Sequence | SDFQVLRAKYRESPLVSAWS EELISKLANDIEATAVRAVR RFGQFHLALSGGSSPVALFQ QLATAHYGFPWAHTHLWLVD ERCVPLSDPESNFQGLQAHL LQHVRIPYYNIHPMPVHLQQ RLCAEEDQGAQIYAREISAL VANSSFDLVLLGMGADGHTA SLFPQSPTGLDGEQLVVLTT SPSQPHRRMSLSLPLINRAK KVAVLVMGRMKREITTLVSR VGHEPKKWPISGVLPHSGQ |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 541-791 of human H6PD/GDH (NP_004276.2). |
Additional Info
| Tissue Specificity | Present in most tissues examined, strongest in liver. |
| Function | Bifunctional enzyme localized in the lumen of the endoplasmic reticulum that catalyzes the first two steps of the oxidative branch of the pentose phosphate pathway/shunt, an alternative to glycolysis and a major source of reducing power and metabolic intermediates for biosynthetic processes. Has a hexose-6-phosphate dehydrogenase activity, with broad substrate specificity compared to glucose-6-phosphate 1-dehydrogenase/G6PD, and catalyzes the first step of the pentose phosphate pathway. In addition, acts as a 6-phosphogluconolactonase and catalyzes the second step of the pentose phosphate pathway. May have a dehydrogenase activity for alternative substrates including glucosamine 6-phosphate and glucose 6-sulfate. The main function of this enzyme is to provide reducing equivalents such as NADPH to maintain the adequate levels of reductive cofactors in the oxidizing environment of the endoplasmic reticulum. By producing NADPH that is needed by reductases of the lumen of the endoplasmic reticulum like corticosteroid 11-beta-dehydrogenase isozyme 1/HSD11B1, indirectly regulates their activity. |
| Protein Name | Gdh/6pgl Endoplasmic Bifunctional Protein Includes - Hexose-6-Phosphate DehydrogenaseGlucose 1-DehydrogenaseGdhGlucose-6-Phosphate Dehydrogenase - 6-Phosphogluconolactonase6pgl |
| Database Links | |
| Cellular Localisation | Endoplasmic Reticulum Lumen |
| Alternative Antibody Names | Anti-Gdh/6pgl Endoplasmic Bifunctional Protein Includes - Hexose-6-Phosphate Dehydrogenase antibodyAnti-Glucose 1-Dehydrogenase antibodyAnti-Gdh antibodyAnti-Glucose-6-Phosphate Dehydrogenase - 6-Phosphogluconolactonase antibodyAnti-6pgl antibodyAnti-H6PD antibodyAnti-GDH antibody |
Information sourced from Uniprot.org