| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Histone H2b Type 1-A is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
H2BC1 |
| Gene ID: |
255626 |
| Uniprot ID: |
H2B1A_HUMAN |
| Immunogen: |
Recombinant protein of human Histone H2B (testis specific). |
| Tissue Specificity | Mainly expressed in testis, and the corresponding protein is also present in mature sperm (at protein level). Also found in some fat cells. |
| Post Translational Modifications | Monoubiquitination at Lys-36 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-122 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons. Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes. Acetylated during spermatogenesis. Acetylated form is most abundant in spermatogonia compared to spermatocytes and round spermatids. Phosphorylated at Thr-117 in spermatogonia, spermatocytes and round spermatids. Methylated at Lys-118 in spermatogonia, spermatocytes and round spermatids. Lactylated in macrophages by EP300/P300 by using lactoyl-CoA directly derived from endogenous or exogenous lactate, leading to stimulates gene transcription. |
| Function | Variant histone specifically required to direct the transformation of dissociating nucleosomes to protamine in male germ cells. Entirely replaces classical histone H2B prior nucleosome to protamine transition and probably acts as a nucleosome dissociating factor that creates a more dynamic chromatin, facilitating the large-scale exchange of histones. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Also found in fat cells, its function and the presence of post-translational modifications specific to such cells are still unclear. |
| Protein Name | Histone H2b Type 1-AHistone H2b - TestisTsh2b.1Htsh2bTestis-Specific Histone H2b |
| Database Links | Reactome: R-HSA-110328Reactome: R-HSA-110329Reactome: R-HSA-110330Reactome: R-HSA-110331Reactome: R-HSA-1221632Reactome: R-HSA-171306Reactome: R-HSA-1912408Reactome: R-HSA-201722Reactome: R-HSA-212300Reactome: R-HSA-2299718Reactome: R-HSA-2559580Reactome: R-HSA-2559582Reactome: R-HSA-2559586Reactome: R-HSA-3214815Reactome: R-HSA-3214847Reactome: R-HSA-427359Reactome: R-HSA-427389Reactome: R-HSA-427413Reactome: R-HSA-5250924Reactome: R-HSA-5334118Reactome: R-HSA-5578749Reactome: R-HSA-5617472Reactome: R-HSA-5625886Reactome: R-HSA-5689880Reactome: R-HSA-5693565Reactome: R-HSA-5693571Reactome: R-HSA-5693607Reactome: R-HSA-606279Reactome: R-HSA-68616Reactome: R-HSA-69473Reactome: R-HSA-73728Reactome: R-HSA-73772Reactome: R-HSA-8866654Reactome: R-HSA-8936459Reactome: R-HSA-8939236Reactome: R-HSA-9018519Reactome: R-HSA-912446Reactome: R-HSA-9609690Reactome: R-HSA-9610379Reactome: R-HSA-9616222Reactome: R-HSA-9670095Reactome: R-HSA-9710421Reactome: R-HSA-977225 |
| Cellular Localisation | NucleusChromosome |
| Alternative Antibody Names | Anti-Histone H2b Type 1-A antibodyAnti-Histone H2b - Testis antibodyAnti-Tsh2b.1 antibodyAnti-Htsh2b antibodyAnti-Testis-Specific Histone H2b antibodyAnti-H2BC1 antibodyAnti-HIST1H2BA antibodyAnti-TSH2B antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
