• Western blot analysis of extracts of various cell lines, using Glycophorin C (GYPC) rabbit monoclonal antibody (STJ11101337) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts from K-562 cells, using Glycophorin C (GYPC) rabbit monoclonal antibody (STJ11101337) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: NA NA NA 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded human placenta using Glycophorin C (GYPC) (GYPC) rabbit monoclonal antibody (STJ11101337) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-GYPC antibody (49-128) [S7MR] (STJ11101337)

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STJ11101337

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Glycophorin C (49-128) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GYPC
Gene ID: 2995
Uniprot ID: GLPC_HUMAN
Immunogen Region: 49-128
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 49-128 of human Glycophorin C (GYPC) (P04921).
Immunogen Sequence: METSTPTIMDIVVIAGVIAA VAIVLVSLLFVMLRYMYRHK GTYHTNEAKGTEFAESADAA LQGDPALQDAGDSSRKEYFI
Tissue Specificity Glycophorin-C is expressed in erythrocytes. Glycophorin-D and IsoGPC are ubiquitously expressed.
Post Translational Modifications O-glycosylated with core 1 or possibly core 8 glycans.
Function This protein is a minor sialoglycoprotein in human erythrocyte membranes. The blood group Gerbich antigens and receptors for Plasmodium falciparum merozoites are most likely located within the extracellular domain. Glycophorin-C plays an important role in regulating the stability of red cells.
Protein Name Glycophorin-C
Glycoconnectin
Glycophorin-D
Gpd
Glycoprotein Beta
Pas-2'
Sialoglycoprotein D
Cd Antigen Cd236
Database Links Reactome: R-HSA-202733
Cellular Localisation Cell Membrane
Single-Pass Type Iii Membrane Protein
Linked To The Membrane Via Band 4
1
Alternative Antibody Names Anti-Glycophorin-C antibody
Anti-Glycoconnectin antibody
Anti-Glycophorin-D antibody
Anti-Gpd antibody
Anti-Glycoprotein Beta antibody
Anti-Pas-2' antibody
Anti-Sialoglycoprotein D antibody
Anti-Cd Antigen Cd236 antibody
Anti-GYPC antibody
Anti-GLPC antibody
Anti-GPC antibody

Information sourced from Uniprot.org

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