• Western blot analysis of extracts of various cell lines, using GYG1 antibody (STJ112256) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-GYG1 antibody (234-333) (STJ112256)

SKU:
STJ112256

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GYG1 (234-333) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GYG1
Gene ID: 2992
Uniprot ID: GLYG_HUMAN
Immunogen Region: 234-333
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 234-333 of human GYG1 (NP_001171649.1).
Immunogen Sequence: EAHDPNMTHPEFLILWWNIF TTNVLPLLQQFGLVKDTCSY VNVEDVSGAISHLSLGEIPA MAQPFVSSEERKERWEQGQA DYMGADSFDNIKRKLDTYLQ
Tissue Specificity Highly expressed in skeletal muscle and heart, with lower levels in brain, lung, kidney and pancreas.
Post Translational Modifications Self-glycosylated by the transfer of glucose residues from UDP-glucose to itself, forming an alpha-1,4-glycan of around 10 residues attached to Tyr-195. Phosphorylated.
Function Self-glucosylates, via an inter-subunit mechanism, to form an oligosaccharide primer that serves as substrate for glycogen synthase.
Protein Name Glycogenin-1
Gn-1
Gn1
Database Links Reactome: R-HSA-3322077
Reactome: R-HSA-3785653
Reactome: R-HSA-3814836
Reactome: R-HSA-3828062
Reactome: R-HSA-5357609
Reactome: R-HSA-6798695
Reactome: R-HSA-70221
Alternative Antibody Names Anti-Glycogenin-1 antibody
Anti-Gn-1 antibody
Anti-Gn1 antibody
Anti-GYG1 antibody
Anti-GYG antibody

Information sourced from Uniprot.org

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