• Immunofluorescence analysis of L929 cells using GUCY1A3 Rabbit polyclonal antibody (STJ114583) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using GUCY1A3 Rabbit polyclonal antibody (STJ114583) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-GUCY1A3 antibody (1-120) (STJ114583)

SKU:
STJ114583

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GUCY1A3 (1-120) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:3000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GUCY1A1
Gene ID: 2982
Uniprot ID: GCYA1_HUMAN
Immunogen Region: 1-120
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human GUCY1A3 (NP_000847.2).
Immunogen Sequence: MFCTKLKDLKITGECPFSLL APGQVPNESSEEAAGSSESC KATVPICQDIPEKNIQESLP QRKTSRSRVYLHTLAESICK LIFPEFERLNVALQRTLAKH KIKESRKSLEREDFEKTIAE
Tissue Specificity Detected in brain cortex and lung (at protein level).
Protein Name Guanylate Cyclase Soluble Subunit Alpha-1
Gcs-Alpha-1
Guanylate Cyclase Soluble Subunit Alpha-3
Gcs-Alpha-3
Soluble Guanylate Cyclase Large Subunit
Database Links Reactome: R-HSA-392154
Reactome: R-HSA-445355
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Guanylate Cyclase Soluble Subunit Alpha-1 antibody
Anti-Gcs-Alpha-1 antibody
Anti-Guanylate Cyclase Soluble Subunit Alpha-3 antibody
Anti-Gcs-Alpha-3 antibody
Anti-Soluble Guanylate Cyclase Large Subunit antibody
Anti-GUCY1A1 antibody
Anti-GUC1A3 antibody
Anti-GUCSA3 antibody
Anti-GUCY1A3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance