• Immunohistochemistry analysis of paraffin-embedded mouse testis using GTPBP4 Rabbit polyclonal antibody (STJ23895) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using GTPBP4 Rabbit polyclonal antibody (STJ23895) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human esophageal using GTPBP4 Rabbit polyclonal antibody (STJ23895) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat ovary using GTPBP4 Rabbit polyclonal antibody (STJ23895) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using GTPBP4 antibody (STJ23895) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-GTPBP4 antibody (385-634) (STJ23895)

SKU:
STJ23895

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GTPBP4 (385-634) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GTPBP4
Gene ID: 23560
Uniprot ID: GTPB4_HUMAN
Immunogen Region: 385-634
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 385-634 of human GTPBP4 (NP_036473.2).
Immunogen Sequence: RKRMETEESRKKRERDLELE MGDDYILDLQKYWDLMNLSE KHDKIPEIWEGHNIADYIDP AIMKKLEELEKEEELRTAAG EYDSVSESEDEEMLEIRQLA KQIREKKKLKILESKEKNTQ GPRMPRTAKKVQRTVLEKEM RSLGVDMDDKDDAHYAVQAR RSRSITRKRKREDSAPPSSV ARSGSCSRTPRDVSGLRDVK MVKKAKTMMKNAQKKMNRLG KKGEADRHVFDMKPKHLLS
Function Involved in the biogenesis of the 60S ribosomal subunit. Acts as TP53 repressor, preventing TP53 stabilization and cell cycle arrest.
Protein Name Gtp-Binding Protein 4
Chronic Renal Failure Gene Protein
Gtp-Binding Protein Ngb
Nucleolar Gtp-Binding Protein 1
Cellular Localisation Nucleus
Nucleolus
Alternative Antibody Names Anti-Gtp-Binding Protein 4 antibody
Anti-Chronic Renal Failure Gene Protein antibody
Anti-Gtp-Binding Protein Ngb antibody
Anti-Nucleolar Gtp-Binding Protein 1 antibody
Anti-GTPBP4 antibody
Anti-CRFG antibody
Anti-NOG1 antibody

Information sourced from Uniprot.org

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