| Short Description : | Mouse monoclonal anti-GTF2H1 for use in WB, ICC, IF and IP in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ICC/IF/IP |
| Host: | Mouse |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | 3E8-D3-H8 |
| Isotype: | IgG1 |
| Conjugation: | Unconjugated |
| Concentration: | 12 mg/mL |
| Purification: | Affinity Purified |
| Dilution Range: | WB 1:500-1:1000IF 1:50-1:200IP 1:20 |
| Formulation: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. |
| Storage Instruction: | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
| Gene Symbol: | GTF2H1 |
| Gene ID: | 2965 |
| Uniprot ID: | TF2H1_HUMAN |
| Immunogen: | Purified recombinant human GTF2H1protein fragments expressed in E.coli. |
| Post Translational Modifications | (Microbial infection) Upon Rift valley fever virus infection, interacts with NSs leading to ubiquitination by the NSs-FBXO3 E3 ligase and proteasome-dependent degradation. |
| Function | Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription. |
| Protein Name | General Transcription Factor Iih Subunit 1Basic Transcription Factor 2 62 Kda SubunitBtf2 P62General Transcription Factor Iih Polypeptide 1Tfiih Basal Transcription Factor Complex P62 Subunit |
| Database Links | Reactome: R-HSA-112382Reactome: R-HSA-113418Reactome: R-HSA-167152Reactome: R-HSA-167158Reactome: R-HSA-167160Reactome: R-HSA-167161Reactome: R-HSA-167162Reactome: R-HSA-167172Reactome: R-HSA-167200Reactome: R-HSA-167246Reactome: R-HSA-427413Reactome: R-HSA-5696395Reactome: R-HSA-5696400Reactome: R-HSA-674695Reactome: R-HSA-6781823Reactome: R-HSA-6781827Reactome: R-HSA-6782135Reactome: R-HSA-6782210Reactome: R-HSA-6796648Reactome: R-HSA-72086Reactome: R-HSA-73762Reactome: R-HSA-73772Reactome: R-HSA-73776Reactome: R-HSA-73779Reactome: R-HSA-73863Reactome: R-HSA-75953Reactome: R-HSA-75955Reactome: R-HSA-76042Reactome: R-HSA-77075 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-General Transcription Factor Iih Subunit 1 antibodyAnti-Basic Transcription Factor 2 62 Kda Subunit antibodyAnti-Btf2 P62 antibodyAnti-General Transcription Factor Iih Polypeptide 1 antibodyAnti-Tfiih Basal Transcription Factor Complex P62 Subunit antibodyAnti-GTF2H1 antibodyAnti-BTF2 antibody |
Information sourced from Uniprot.org
