Anti-GTF2H1 antibody [3E8-D3-H8] (STJ99084)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
ImmunogenPurified recombinant human GTF2H1protein fragments expressed in E.coli.
General Information
| Short Description | Mouse monoclonal anti-General transcription factor IIH subunit 1 for use in WB, IP and ICC in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IP/ICC |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 3E8-D3-H8 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:300ICC 1:50 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GTF2H1 |
| Gene ID | 2965 |
| Uniprot ID | TF2H1_HUMAN |
| Immunogen | Purified recombinant human GTF2H1protein fragments expressed in E.coli. |
| Specificity | This antibody detects endogenous levels of GTF2H1 and does not cross-react with related proteins. |
Additional Info
| Function | Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription. |
| Protein Name | General Transcription Factor Iih Subunit 1Basic Transcription Factor 2 62 Kda SubunitBtf2 P62General Transcription Factor Iih Polypeptide 1Tfiih Basal Transcription Factor Complex P62 Subunit |
| Database Links | Reactome: R-HSA-112382Reactome: R-HSA-113418Reactome: R-HSA-167152Reactome: R-HSA-167158Reactome: R-HSA-167160Reactome: R-HSA-167161Reactome: R-HSA-167162Reactome: R-HSA-167172Reactome: R-HSA-167200Reactome: R-HSA-167246Reactome: R-HSA-427413Reactome: R-HSA-5696395Reactome: R-HSA-5696400Reactome: R-HSA-674695Reactome: R-HSA-6781823Reactome: R-HSA-6781827Reactome: R-HSA-6782135Reactome: R-HSA-6782210Reactome: R-HSA-6796648Reactome: R-HSA-72086Reactome: R-HSA-73762Reactome: R-HSA-73772Reactome: R-HSA-73776Reactome: R-HSA-73779Reactome: R-HSA-73863Reactome: R-HSA-75953Reactome: R-HSA-75955Reactome: R-HSA-76042Reactome: R-HSA-77075 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-General Transcription Factor Iih Subunit 1 antibodyAnti-Basic Transcription Factor 2 62 Kda Subunit antibodyAnti-Btf2 P62 antibodyAnti-General Transcription Factor Iih Polypeptide 1 antibodyAnti-Tfiih Basal Transcription Factor Complex P62 Subunit antibodyAnti-GTF2H1 antibodyAnti-BTF2 antibody |
Information sourced from Uniprot.org