• Western blot analysis of extracts of various cell lines, using GTF2E2 antibody (STJ111392) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of various cell lines, using GTF2E2 antibody (STJ111392) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-GTF2E2 antibody (1-291) (STJ111392)

SKU:
STJ111392

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GTF2E2 (1-291) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GTF2E2
Gene ID: 2961
Uniprot ID: T2EB_HUMAN
Immunogen Region: 1-291
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-291 of human GTF2E2 (NP_002086.1).
Immunogen Sequence: MDPSLLRERELFKKRALSTP VVEKRSASSESSSSSSKKKK TKVEHGGSSGSKQNSDHSNG SFNLKALSGSSGYKFGVLAK IVNYMKTRHQRGDTHPLTLD EILDETQHLDIGLKQKQWLM TEALVNNPKIEVIDGKYAFK PKYNVRDKKALLRLLDQHDQ RGLGGILLEDIEEALPNSQK AVKALGDQILFVNRPDKKKI LFFNDKSCQFSVDEEFQKLW RSVTVDSMDEEKIEEYLKR
Function Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.
Protein Name Transcription Initiation Factor Iie Subunit Beta
Tfiie-Beta
General Transcription Factor Iie Subunit 2
Database Links Reactome: R-HSA-167161
Reactome: R-HSA-167162
Reactome: R-HSA-167172
Reactome: R-HSA-674695
Reactome: R-HSA-6807505
Reactome: R-HSA-73776
Reactome: R-HSA-73779
Reactome: R-HSA-75953
Reactome: R-HSA-76042
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Transcription Initiation Factor Iie Subunit Beta antibody
Anti-Tfiie-Beta antibody
Anti-General Transcription Factor Iie Subunit 2 antibody
Anti-GTF2E2 antibody
Anti-TF2E2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance