• Immunohistochemistry analysis of paraffin-embedded human colon using TFIIB monoclonal antibody.
  • Immunofluorescence analysis of HeLa cells using TFIIB monoclonal antibody.
  • Western blot analysis using TFIIB monoclonal antibody against Jurkat, MCF7, HeLa cell lysate.

Anti-GTF2B antibody (STJ98560)

SKU:
STJ98560

Current Stock:
Host: Mouse
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat/Cow/Chicken/Dog/Pig
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Transcription initiation factor IIB is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Conjugation: Unconjugated
Formulation: Liquid in 0.1M Tris-Glycine (pH7.4, 150 mM NaCl) , 0.2% Sodium Azide and 50% Glycerol.
Purification: Affinity purification
Concentration: 1 mg/mL
Dilution Range: WB 1:1000-1:2000
IHC 1:500-1:1000
IF 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GTF2B
Gene ID: 2959
Uniprot ID: TF2B_HUMAN
Specificity: TFIIB Monoclonal Antibody detects endogenous levels of TFIIB protein.
Immunogen: Purified recombinant human TFIIB protein fragments expressed in E.coli.
Post Translational Modifications Acetylated. Autoacetylated.autoacetylation at Lys-238 stimulates transcription activation.
Function General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA. Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex. Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle. Associates with chromatin to core promoter-specific regions. Binds to two distinct DNA core promoter consensus sequence elements in a TBP-independent manner.these IIB-recognition elements (BREs) are localized immediately upstream (BREu), 5'-GCGCGACGCC-3', and downstream (BREd), 5'-GATTGATGGTTGTG-3', of the TATA box element. Modulates transcription start site selection. Exhibits also autoacetyltransferase activity that contributes to the activated transcription.
Protein Name Transcription Initiation Factor Iib
General Transcription Factor Tfiib
S300-Ii
Database Links Reactome: R-HSA-167161
Reactome: R-HSA-167162
Reactome: R-HSA-167172
Reactome: R-HSA-674695
Reactome: R-HSA-6807505
Reactome: R-HSA-73776
Reactome: R-HSA-73779
Reactome: R-HSA-75953
Reactome: R-HSA-76042
Cellular Localisation Nucleus
Chromosome
Non-Acetylated Form Colocalizes With Dna In The G0/1
S And G2 Phases Of The Cell Cycle
But Not During Mitosis
Acetylated Form Colocalizes At Transcriptionally Silent Mitotic Chromatids During Mitosis At Metaphase
Anaphase
And Telophase Phases Of The Cell Cycle
Alternative Antibody Names Anti-Transcription Initiation Factor Iib antibody
Anti-General Transcription Factor Tfiib antibody
Anti-S300-Ii antibody
Anti-GTF2B antibody
Anti-TF2B antibody
Anti-TFIIB antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance