![Western blot analysis of lysates from wild type (WT) and GSTO1 knockout (KO) 293T cells, using [KO Validated] GSTO1 Rabbit polyclonal antibody (STJ11100768) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/89705/358188/STJ11100768_1__90554.1713122502.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and GSTO1 knockout (KO) 293T cells, using [KO Validated] GSTO1 Rabbit polyclonal antibody (STJ11100768) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Western blot analysis of lysates from wild type (WT) and GSTO1 knockout (KO) 293T cells, using [KO Validated] GSTO1 Rabbit polyclonal antibody (STJ11100768) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/89705/358188/STJ11100768_1__90554.1713122502.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and GSTO1 knockout (KO) 293T cells, using [KO Validated] GSTO1 Rabbit polyclonal antibody (STJ11100768) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
Anti-GSTO1 antibody (1-241) (STJ11100768)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal GSTO1 (1-241) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:200-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GSTO1 |
| Gene ID | 9446 |
| Uniprot ID | GSTO1_HUMAN |
| Immunogen Region | 1-241 |
| Immunogen Sequence | MSGESARSLGKGSAPPGPVP EGSIRIYSMRFCPFAERTRL VLKAKGIRHEVININLKNKP EWFFKKNPFGLVPVLENSQG QLIYESAITCEYLDEAYPGK KLLPDDPYEKACQKMILELF SKVPSLVGSFIRSQNKEDYA GLKEEFRKEFTKLEEVLTNK KTTFFGGNSISMIDYLIWPW FERLEAMKLNECVDHTPKLK LWMAAMKEDPTVSALLTSEK DWQGFLELYLQNSPEACDY |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-241 of human GSTO1 (NP_004823.1). |
Additional Info
| Tissue Specificity | Ubiquitous. Highest expression in liver, pancreas, skeletal muscle, spleen, thymus, colon, blood leukocyte and heart. Lowest expression in brain, placenta and lung. |
| Function | Exhibits glutathione-dependent thiol transferase and dehydroascorbate reductase activities. Has S-(phenacyl)glutathione reductase activity. Also has glutathione S-transferase activity. Participates in the biotransformation of inorganic arsenic and reduces monomethylarsonic acid (MMA) and dimethylarsonic acid. |
| Protein Name | Glutathione S-Transferase Omega-1Gsto-1Glutathione S-Transferase Omega 1-1Gsto 1-1Glutathione-Dependent Dehydroascorbate ReductaseMonomethylarsonic Acid ReductaseMma(V ReductaseS-(Phenacylglutathione ReductaseSpg-R |
| Database Links | Reactome: R-HSA-156581Reactome: R-HSA-156590Reactome: R-HSA-196836Reactome: R-HSA-8950505 |
| Cellular Localisation | CytoplasmCytosol |
| Alternative Antibody Names | Anti-Glutathione S-Transferase Omega-1 antibodyAnti-Gsto-1 antibodyAnti-Glutathione S-Transferase Omega 1-1 antibodyAnti-Gsto 1-1 antibodyAnti-Glutathione-Dependent Dehydroascorbate Reductase antibodyAnti-Monomethylarsonic Acid Reductase antibodyAnti-Mma(V Reductase antibodyAnti-S-(Phenacylglutathione Reductase antibodyAnti-Spg-R antibodyAnti-GSTO1 antibodyAnti-GSTTLP28 antibody |
Information sourced from Uniprot.org