• Immunofluorescence analysis of U-2 OS cells using GSTA1 Rabbit polyclonal antibody (STJ11100222) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using GSTA1 Rabbit polyclonal antibody (STJ11100222) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using GSTA1 Rabbit polyclonal antibody (STJ11100222) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using GSTA1 Rabbit polyclonal antibody (STJ11100222) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-GSTA1 antibody (1-222) (STJ11100222)

SKU:
STJ11100222

€140.63 - €653.13
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GSTA1 (1-222) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GSTA1
Gene ID: 2938
Uniprot ID: GSTA1_HUMAN
Immunogen Region: 1-222
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-222 of human GSTA1 (NP_665683.1).
Immunogen Sequence: MAEKPKLHYFNARGRMESTR WLLAAAGVEFEEKFIKSAED LDKLRNDGYLMFQQVPMVEI DGMKLVQTRAILNYIASKYN LYGKDIKERALIDMYIEGIA DLGEMILLLPVCPPEEKDAK LALIKEKIKNRYFPAFEKVL KSHGQDYLVGNKLSRADIHL VELLYYVEELDSSLISSFPL LKALKTRISNLPTVKKFLQP GSPRKPPMDEKSLEEARKIF RF
Tissue Specificity Liver.
Function Glutathione S-transferase that catalyzes the nucleophilic attack of the sulfur atom of glutathione on the electrophilic groups of a wide range of exogenous and endogenous compounds (Probable). Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2). It also catalyzes the isomerization of D5-androstene-3,17-dione (AD) into D4-androstene-3,17-dione and may therefore play an important role in hormone biosynthesis. Through its glutathione-dependent peroxidase activity toward the fatty acid hydroperoxide (13S)-hydroperoxy-(9Z,11E)-octadecadienoate/13-HPODE it is also involved in the metabolism of oxidized linoleic acid.
Protein Name Glutathione S-Transferase A1
13-Hydroperoxyoctadecadienoate Peroxidase
Androst-5-Ene-3 -17-Dione Isomerase
Gst Ha Subunit 1
Gst Class-Alpha Member 1
Gst-Epsilon
Gsta1-1
Gth1 Cleaved Into - Glutathione S-Transferase A1 - N-Terminally Processed
Database Links Reactome: R-HSA-156590
Reactome: R-HSA-189483
Reactome: R-HSA-9748787
Reactome: R-HSA-9818027
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Glutathione S-Transferase A1 antibody
Anti-13-Hydroperoxyoctadecadienoate Peroxidase antibody
Anti-Androst-5-Ene-3 -17-Dione Isomerase antibody
Anti-Gst Ha Subunit 1 antibody
Anti-Gst Class-Alpha Member 1 antibody
Anti-Gst-Epsilon antibody
Anti-Gsta1-1 antibody
Anti-Gth1 Cleaved Into - Glutathione S-Transferase A1 - N-Terminally Processed antibody
Anti-GSTA1 antibody

Information sourced from Uniprot.org

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