Western blot analysis of various lysates, using GSPT1 Rabbit polyclonal antibody (STJ118654) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-GSPT1 antibody (138-420) (STJ118654)

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STJ118654
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GSPT1
Gene ID: 2935
Uniprot ID: ERF3A_HUMAN
Immunogen Region: 138-420
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 138-420 of human GSPT1 (NP_001123478.1).
Immunogen Sequence: SMELSEPIENGETEMSPEES WEHKEEISEAEPGGGSLGDG RPPEESAHEMMEEEEEIPKP KSVVAPPGAPKKEHVNVVFI GHVDAGKSTIGGQIMYLTGM VDKRTLEKYEREAKEKNRET WYLSWALDTNQEERDKGKTV EVGRAYFETEKKHFTILDAP GHKSFVPNMIGGASQADLAV LVISARKGEFETGFEKGGQT REHAMLAKTAGVKHLIVLIN KMDDPTVNWSNERYEECKE
Function GTPase component of the eRF1-eRF3-GTP ternary complex, a ternary complex that mediates translation termination in response to the termination codons UAA, UAG and UGA. GSPT1/ERF3A mediates ETF1/ERF1 delivery to stop codons: The eRF1-eRF3-GTP complex binds to a stop codon in the ribosomal A-site. GTP hydrolysis by GSPT1/ERF3A induces a conformational change that leads to its dissociation, permitting ETF1/ERF1 to accommodate fully in the A-site. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Required for SHFL-mediated translation termination which inhibits programmed ribosomal frameshifting (-1PRF) of mRNA from viruses and cellular genes.
Protein Name Eukaryotic Peptide Chain Release Factor Gtp-Binding Subunit Erf3a
Eukaryotic Peptide Chain Release Factor Subunit 3a
Erf3a
G1 To S Phase Transition Protein 1 Homolog
Database Links Reactome: R-HSA-72764
Reactome: R-HSA-9010553
Reactome: R-HSA-975956
Reactome: R-HSA-975957
Alternative Antibody Names Anti-Eukaryotic Peptide Chain Release Factor Gtp-Binding Subunit Erf3a antibody
Anti-Eukaryotic Peptide Chain Release Factor Subunit 3a antibody
Anti-Erf3a antibody
Anti-G1 To S Phase Transition Protein 1 Homolog antibody
Anti-GSPT1 antibody
Anti-ERF3A antibody

Information sourced from Uniprot.org

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