• Immunohistochemistry analysis of paraffin-embedded mouse brain using Gelsolin Rabbit monoclonal antibody (STJ11101830) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using Gelsolin Rabbit monoclonal antibody (STJ11101830) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Western blot analysis of extracts of various cell lines, using Gelsolin Rabbit monoclonal antibody (STJ11101830) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-GSN antibody (400-500) [S0MR] (STJ11101830)

SKU:
STJ11101830

€183.13 - €799.38
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Gelsolin (400-500) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GSN
Gene ID: 2934
Uniprot ID: GELS_HUMAN
Immunogen Region: 400-500
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 400-500 of human Gelsolin (NP_000168.1).
Immunogen Sequence: DQTDGLGLSYLSSHIANVER VPFDAATLHTSTAMAAQHGM DDDGTGQKQIWRIEGSNKVP VDPATYGQFYGGDSYIILYN YRHGGRQGQIIYNWQGAQST Q
Tissue Specificity Phagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells.
Post Translational Modifications Phosphorylation on Tyr-86, Tyr-409, Tyr-465, Tyr-603 and Tyr-651 in vitro is induced in presence of phospholipids.
Function Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.
Protein Name Gelsolin
Agel
Actin-Depolymerizing Factor
Adf
Brevin
Database Links Reactome: R-HSA-264870
Reactome: R-HSA-6798695
Reactome: R-HSA-9662361
Reactome: R-HSA-977225
Cellular Localisation Isoform 2: Cytoplasm
Cytoskeleton
Isoform 1: Secreted
Alternative Antibody Names Anti-Gelsolin antibody
Anti-Agel antibody
Anti-Actin-Depolymerizing Factor antibody
Anti-Adf antibody
Anti-Brevin antibody
Anti-GSN antibody

Information sourced from Uniprot.org

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