• Immunofluorescence analysis of U-2 OS cells using Gelsolin Rabbit polyclonal antibody (STJ11101188) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using Gelsolin Rabbit polyclonal antibody (STJ11101188) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using Gelsolin Rabbit polyclonal antibody (STJ11101188) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded rat lung using Gelsolin Rabbit polyclonal antibody (STJ11101188) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse spleen using Gelsolin Rabbit polyclonal antibody (STJ11101188) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using Gelsolin Rabbit polyclonal antibody (STJ11101188) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using Gelsolin Rabbit polyclonal antibody (STJ11101188) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using Gelsolin antibody (STJ11101188) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-GSN antibody (282-438) (STJ11101188)

SKU:
STJ11101188

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GSN (282-438) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GSN
Gene ID: 2934
Uniprot ID: GELS_HUMAN
Immunogen Region: 282-438
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 282-438 of human Gelsolin (NP_000168.1).
Immunogen Sequence: AGTEDTAKEDAANRKLAKLY KVSNGAGTMSVSLVADENPF AQGALKSEDCFILDHGKDGK IFVWKGKQANTEERKAALKT ASDFITKMDYPKQTQVSVLP EGGETPLFKQFFKNWRDPDQ TDGLGLSYLSSHIANVERVP FDAATLHTSTAMAAQHG
Tissue Specificity Phagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells.
Post Translational Modifications Phosphorylation on Tyr-86, Tyr-409, Tyr-465, Tyr-603 and Tyr-651 in vitro is induced in presence of phospholipids.
Function Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.
Protein Name Gelsolin
Agel
Actin-Depolymerizing Factor
Adf
Brevin
Database Links Reactome: R-HSA-264870
Reactome: R-HSA-6798695
Reactome: R-HSA-9662361
Reactome: R-HSA-977225
Cellular Localisation Isoform 2: Cytoplasm
Cytoskeleton
Isoform 1: Secreted
Alternative Antibody Names Anti-Gelsolin antibody
Anti-Agel antibody
Anti-Actin-Depolymerizing Factor antibody
Anti-Adf antibody
Anti-Brevin antibody
Anti-GSN antibody

Information sourced from Uniprot.org

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