• Immunofluorescence analysis of NIH/3T3 cells using Granulin Rabbit polyclonal antibody (STJ114314) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of A-431 cells, using Granulin antibody (STJ114314) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-GRN antibody (20-593) (STJ114314)

SKU:
STJ114314

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GRN (20-593) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GRN
Gene ID: 2896
Uniprot ID: GRN_HUMAN
Immunogen Region: 20-593
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-593 of human Granulin (NP_002078.1).
Immunogen Sequence: CPDGQFCPVACCLDPGGASY SCCRPLLDKWPTTLSRHLGG PCQVDAHCSAGHSCIFTVSG TSSCCPFPEAVACGDGHHCC PRGFHCSADGRSCFQRSGNN SVGAIQCPDSQFECPDFSTC CVMVDGSWGCCPMPQASCCE DRVHCCPHGAFCDLVHTRCI TPTGTHPLAKKLPAQRTNRA VALSSSVMCPDARSRCPDGS TCCELPSGKYGCCPMPNATC CSDHLHCCPQDTVCDLIQS
Tissue Specificity In myelogenous leukemic cell lines of promonocytic, promyelocytic, and proerythroid lineage, in fibroblasts, and very strongly in epithelial cell lines. Present in inflammatory cells and bone marrow. Highest levels in kidney.
Post Translational Modifications Cleaved by ELANE.proteolysis is blocked by SLPI and is concentration- and time-dependent and induces CXCL8/IL-8 production.granulin-3 and granulin-4 are resistant to ELANE. Cleaved by CTSL in lysosome thus regulating the maturation and turnover of progranulin within the lysosome.
Function Secreted protein that acts as a key regulator of lysosomal function and as a growth factor involved in inflammation, wound healing and cell proliferation. Regulates protein trafficking to lysosomes and, also the activity of lysosomal enzymes. Facilitates also the acidification of lysosomes, causing degradation of mature CTSD by CTSB. In addition, functions as wound-related growth factor that acts directly on dermal fibroblasts and endothelial cells to promote division, migration and the formation of capillary-like tubule structures. Also promotes epithelial cell proliferation by blocking TNF-mediated neutrophil activation preventing release of oxidants and proteases. Moreover, modulates inflammation in neurons by preserving neurons survival, axonal outgrowth and neuronal integrity. Granulin-4: Promotes proliferation of the epithelial cell line A431 in culture. Granulin-3: Inhibits epithelial cell proliferation and induces epithelial cells to secrete IL-8. Granulin-7: Stabilizes CTSD through interaction with CTSD leading to maintain its aspartic-type peptidase activity.
Protein Name Progranulin
Pgrn
Acrogranin
Epithelin Precursor
Glycoprotein Of 88 Kda
Gp88
Glycoprotein 88
Granulin Precursor
Pc Cell-Derived Growth Factor
Pcdgf
Proepithelin
Pepi Cleaved Into - Paragranulin - Granulin-1
Granulin G - Granulin-2
Granulin F - Granulin-3
Epithelin-2
Granulin B - Granulin-4
Epithelin-1
Granulin A - Granulin-5
Granulin C - Granulin-6
Granulin D - Granulin-7
Granulin E
Database Links Reactome: R-HSA-6798695
Cellular Localisation Secreted
Lysosome
Endocytosed By Sort1 And Delivred To Lysosomes
Targeted To Lysosome By Psap Via M6pr And Lrp1
In Both Biosynthetic And Endocytic Pathways
Co-Localized With Gba1 In The Intracellular Trafficking Compartments Until To Lysosome
Alternative Antibody Names Anti-Progranulin antibody
Anti-Pgrn antibody
Anti-Acrogranin antibody
Anti-Epithelin Precursor antibody
Anti-Glycoprotein Of 88 Kda antibody
Anti-Gp88 antibody
Anti-Glycoprotein 88 antibody
Anti-Granulin Precursor antibody
Anti-Pc Cell-Derived Growth Factor antibody
Anti-Pcdgf antibody
Anti-Proepithelin antibody
Anti-Pepi Cleaved Into - Paragranulin - Granulin-1 antibody
Anti-Granulin G - Granulin-2 antibody
Anti-Granulin F - Granulin-3 antibody
Anti-Epithelin-2 antibody
Anti-Granulin B - Granulin-4 antibody
Anti-Epithelin-1 antibody
Anti-Granulin A - Granulin-5 antibody
Anti-Granulin C - Granulin-6 antibody
Anti-Granulin D - Granulin-7 antibody
Anti-Granulin E antibody
Anti-GRN antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance