• Immunohistochemistry analysis of paraffin-embedded rat retina using GRK1 antibody (STJ23869) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using GRK1 antibody (STJ23869) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-GRK1 antibody (1-160) (STJ23869)

SKU:
STJ23869

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GRK1
Gene ID: 6011
Uniprot ID: GRK1_HUMAN
Immunogen Region: 1-160
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human GRK1 (NP_002920.1).
Immunogen Sequence: MDFGSLETVVANSAFIAARG SFDGSSSQPSRDKKYLAKLK LPPLSKCESLRDSLSLEFES VCLEQPIGKKLFQQFLQSAE KHLPALELWKDIEDYDTADN DLQPQKAQTILAQYLDPQAK LFCSFLDEGIVAKFKEGPVE IQDGLFQPLLQATLAHLGQA
Tissue Specificity Retinal-specific. Expressed in rods and cones cells.
Post Translational Modifications Autophosphorylated, Ser-21 is a minor site of autophosphorylation compared to Ser-491 and Thr-492. Phosphorylation at Ser-21 is regulated by light and activated by cAMP. Farnesylation is required for full activity.
Function Retina-specific kinase involved in the signal turnoff via phosphorylation of rhodopsin (RHO), the G protein- coupled receptor that initiates the phototransduction cascade. This rapid desensitization is essential for scotopic vision and permits rapid adaptation to changes in illumination. May play a role in the maintenance of the outer nuclear layer in the retina.
Protein Name Rhodopsin Kinase Grk1
Rk
G Protein-Coupled Receptor Kinase 1
Database Links Reactome: R-HSA-2514859
Cellular Localisation Membrane
Lipid-Anchor
Cell Projection
Cilium
Photoreceptor Outer Segment
Subcellular Location Is Not Affected By Light Or Dark Conditions
Alternative Antibody Names Anti-Rhodopsin Kinase Grk1 antibody
Anti-Rk antibody
Anti-G Protein-Coupled Receptor Kinase 1 antibody
Anti-GRK1 antibody
Anti-RHOK antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance