• Western blot analysis of lysates from human fetal intestine, using GRB14 Rabbit polyclonal antibody (STJ23855). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-GRB14 antibody (1-180) (STJ23855)

SKU:
STJ23855

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GRB14 (1-180) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GRB14
Gene ID: 2888
Uniprot ID: GRB14_HUMAN
Immunogen Region: 1-180
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human GRB14 (NP_004481.2).
Immunogen Sequence: MTTSLQDGQSAASRAAARDS PLAAQVCGAAQGRGDAHDLA PAPWLHARALLPLPDGTRGC AADRRKKKDLDVPEMPSIPN PFPELCCSPFTSVLSADLFP KANSRKKQVIKVYSEDETSR ALDVPSDITARDVCQLLILK NHYIDDHSWTLFEHLPHIGV ERTIEDHELVIEVLSNWGIE
Tissue Specificity Expressed at high levels in the liver, kidney, pancreas, testis, ovary, heart and skeletal muscle.
Post Translational Modifications Phosphorylated on serine residues. Phosphorylated on tyrosine residues by TEK/TIE2.
Function Adapter protein which modulates coupling of cell surface receptor kinases with specific signaling pathways. Binds to, and suppresses signals from, the activated insulin receptor (INSR). Potent inhibitor of insulin-stimulated MAPK3 phosphorylation. Plays a critical role regulating PDPK1 membrane translocation in response to insulin stimulation and serves as an adapter protein to recruit PDPK1 to activated insulin receptor, thus promoting PKB/AKT1 phosphorylation and transduction of the insulin signal.
Protein Name Growth Factor Receptor-Bound Protein 14
Grb14 Adapter Protein
Database Links Reactome: R-HSA-210993
Cellular Localisation Cytoplasm
Endosome Membrane
Peripheral Membrane Protein
Upon Insulin Stimulation
Translocates To The Plasma Membrane
Alternative Antibody Names Anti-Growth Factor Receptor-Bound Protein 14 antibody
Anti-Grb14 Adapter Protein antibody
Anti-GRB14 antibody

Information sourced from Uniprot.org

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