| Short Description : | Rabbit polyclonal GRB14 (1-180) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | GRB14 |
| Gene ID: | 2888 |
| Uniprot ID: | GRB14_HUMAN |
| Immunogen Region: | 1-180 |
| Immunogen Sequence: | MTTSLQDGQSAASRAAARDS PLAAQVCGAAQGRGDAHDLA PAPWLHARALLPLPDGTRGC AADRRKKKDLDVPEMPSIPN PFPELCCSPFTSVLSADLFP KANSRKKQVIKVYSEDETSR ALDVPSDITARDVCQLLILK NHYIDDHSWTLFEHLPHIGV ERTIEDHELVIEVLSNWGIE |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human GRB14 (NP_004481.2). |
| Tissue Specificity | Expressed at high levels in the liver, kidney, pancreas, testis, ovary, heart and skeletal muscle. |
| Post Translational Modifications | Phosphorylated on serine residues. Phosphorylated on tyrosine residues by TEK/TIE2. |
| Function | Adapter protein which modulates coupling of cell surface receptor kinases with specific signaling pathways. Binds to, and suppresses signals from, the activated insulin receptor (INSR). Potent inhibitor of insulin-stimulated MAPK3 phosphorylation. Plays a critical role regulating PDPK1 membrane translocation in response to insulin stimulation and serves as an adapter protein to recruit PDPK1 to activated insulin receptor, thus promoting PKB/AKT1 phosphorylation and transduction of the insulin signal. |
| Protein Name | Growth Factor Receptor-Bound Protein 14Grb14 Adapter Protein |
| Database Links | Reactome: R-HSA-210993 |
| Cellular Localisation | CytoplasmEndosome MembranePeripheral Membrane ProteinUpon Insulin StimulationTranslocates To The Plasma Membrane |
| Alternative Antibody Names | Anti-Growth Factor Receptor-Bound Protein 14 antibodyAnti-Grb14 Adapter Protein antibodyAnti-GRB14 antibody |
Information sourced from Uniprot.org
![Confocal immunofluorescence analysis of HeLa cells using [KO Validated] HNRNPA2B1 Rabbit polyclonal antibody (STJ24063) at dilution of 1:400. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/99826/385443/STJ24063_1__97324.1713154337.jpg?c=1 "Confocal immunofluorescence analysis of HeLa cells using [KO Validated] HNRNPA2B1 Rabbit polyclonal antibody (STJ24063) at dilution of 1:400. Blue: DAPI for nuclear staining.")
