Anti-GRAB antibody (STJA0010180)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from mouse GRAB
General Information
| Short Description | Rabbit polyclonal anti-GRAB for use in WB and ELISA in Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:1000-2000ELISA 1:5000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | Gzmb |
| Gene ID | 14939 |
| Uniprot ID | GRAB_MOUSE |
| Immunogen | Synthesized peptide derived from mouse GRAB |
| Specificity | This antibody detects endogenous levels of Mouse GRAB |
Additional Info
| Function | Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse. It cleaves after Asp. Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3 and -9 (CASP3 and CASP9, respectively) to give rise to active enzymes mediating apoptosis. Cleaves and activates CASP7 in response to bacterial infection, promoting plasma membrane repair. |
| Protein Name | Granzyme B(G -HCtla-1Cytotoxic Cell Protease 1Ccp1Fragmentin-2 |
| Database Links | Reactome: R-MMU-5620971Reactome: -MMU-75108 |
| Cellular Localisation | SecretedCytolytic GranuleDelivered Into The Target Cell By Perforin |
| Alternative Antibody Names | Anti-Granzyme B(G -H antibodyAnti-Ctla-1 antibodyAnti-Cytotoxic Cell Protease 1 antibodyAnti-Ccp1 antibodyAnti-Fragmentin-2 antibodyAnti-Gzmb antibodyAnti-Ctla-1 antibodyAnti-Ctla1 antibody |
Information sourced from Uniprot.org