• Immunofluorescence analysis of 293T cells using GPX1 Rabbit polyclonal antibody (STJ23851) at dilution of 1:200. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of THP-1 cells using GPX1 Rabbit polyclonal antibody (STJ23851) at dilution of 1:200. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using GPX1 Rabbit polyclonal antibody (STJ23851) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Negative control (NC) : MCF7. Exposuretime: 60s.

Anti-GPX1 antibody (100-200) (STJ23851)

SKU:
STJ23851

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GPX1 (100-200) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GPX1
Gene ID: 2876
Uniprot ID: GPX1_HUMAN
Immunogen Region: 100-200
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human GPX1 (NP_000572.2).
Immunogen Sequence: RPGGGFEPNFMLFEKCEVNG AGAHPLFAFLREALPAPSDD ATALMTDPKLITWSPVCRND VAWNFEKFLVGPDGVPLRRY SRRFQTIDIEPDIEALLSQG P
Tissue Specificity Expressed in platelets (at protein level).
Post Translational Modifications During periods of oxidative stress, Sec-49 may react with a superoxide radical, irreversibly lose hydroselenide and be converted to dehydroalanine.
Function Protects the hemoglobin in erythrocytes from oxidative breakdown. In platelets, plays a crucial role of glutathione peroxidase in the arachidonic acid metabolism.
Protein Name Glutathione Peroxidase 1
Gpx-1
Gshpx-1
Cellular Glutathione Peroxidase
Database Links Reactome: R-HSA-2142688
Reactome: R-HSA-2142712
Reactome: R-HSA-2142770
Reactome: R-HSA-3299685
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Glutathione Peroxidase 1 antibody
Anti-Gpx-1 antibody
Anti-Gshpx-1 antibody
Anti-Cellular Glutathione Peroxidase antibody
Anti-GPX1 antibody

Information sourced from Uniprot.org

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