• Western blot analysis of extracts of various cell lines, using GPT antibody (STJ23848) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-GPT antibody (1-190) (STJ23848)

SKU:
STJ23848

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GPT (1-190) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GPT
Gene ID: 2875
Uniprot ID: ALAT1_HUMAN
Immunogen Region: 1-190
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human GPT (NP_005300.1).
Immunogen Sequence: MASSTGDRSQAVRHGLRAKV LTLDGMNPRVRRVEYAVRGP IVQRALELEQELRQGVKKPF TEVIRANIGDAQAMGQRPIT FLRQVLALCVNPDLLSSPNF PDDAKKRAERILQACGGHSL GAYSVSSGIQLIREDVARYI ERRDGGIPADPNNVFLSTGA SDAIVTVLKLLVAGEGHTRT GVLIPIPQYP
Tissue Specificity Liver, kidney, heart, and skeletal muscles. Expressed at moderate levels in the adipose tissue.
Function Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles.
Protein Name Alanine Aminotransferase 1
Alt1
Glutamate Pyruvate Transaminase 1
Gpt 1
Glutamic--Alanine Transaminase 1
Glutamic--Pyruvic Transaminase 1
Database Links Reactome: R-HSA-8964540
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Alanine Aminotransferase 1 antibody
Anti-Alt1 antibody
Anti-Glutamate Pyruvate Transaminase 1 antibody
Anti-Gpt 1 antibody
Anti-Glutamic--Alanine Transaminase 1 antibody
Anti-Glutamic--Pyruvic Transaminase 1 antibody
Anti-GPT antibody
Anti-AAT1 antibody
Anti-GPT1 antibody

Information sourced from Uniprot.org

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