Anti-GPR35 antibody (50-150) (STJ119935)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal GPR35 (50-150) antibody for use in WB and ELISA in mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GPR35 |
| Gene ID | 2859 |
| Uniprot ID | GPR35_HUMAN |
| Immunogen Region | 50-150 |
| Immunogen Sequence | QQWTETRIYMTNLAVADLCL LCTLPFVLHSLRDTSDTPLC QLSQGIYLTNRYMSISLVTA IAVDRYVAVRHPLRARGLRS PRQAAAVCAVLWVLVIGSLV A |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 50-150 of human GPR35 (NP_005292.2). |
Additional Info
| Tissue Specificity | Predominantly expressed in immune and gastrointestinal tissues. |
| Post Translational Modifications | Multiply phosphorylated in clusters of serines and threonines in the C-terminal tail. Phosphorylation of Ser-300 and Ser-303 is mediated by GRK5 and/or GRK6. |
| Function | G-protein coupled receptor that binds to several ligands including the tryptophan metabolite kynurenic acid (KYNA), lysophosphatidic acid (LPA) or 5-hydroxyindoleacetic acid (5-HIAA) with high affinity, leading to rapid and transient activation of numerous intracellular signaling pathways. Plays a role in neutrophil recruitment to sites of inflammation and bacterial clearance through the major serotonin metabolite 5-HIAA that acts as a physiological ligand. Stimulates lipid metabolism, thermogenic, and anti-inflammatory gene expression in adipose tissue once activated by kynurenic acid. In macrophages, activation by lysophosphatidic acid promotes GPR35-induced signaling with a distinct transcriptional profile characterized by TNF production associated with ERK and NF-kappa-B activation. In turn, induces chemotaxis of macrophages. |
| Protein Name | G-Protein Coupled Receptor 35Kynurenic Acid ReceptorKyna Receptor |
| Database Links | Reactome: R-HSA-373076 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinInternalized To The Cytoplasm After Exposure To Kynurenic Acid |
| Alternative Antibody Names | Anti-G-Protein Coupled Receptor 35 antibodyAnti-Kynurenic Acid Receptor antibodyAnti-Kyna Receptor antibodyAnti-GPR35 antibody |
Information sourced from Uniprot.org