• Western blot analysis of extracts of U-87MG cells, using GPM6A antibody (STJ111550) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of paraffin-embedded human stomach using GPM6A antibody (STJ111550) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse stomach using GPM6A antibody (STJ111550) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded mouse stomach using GPM6A antibody (STJ111550) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human stomach using GPM6A antibody (STJ111550) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of U-87MG cells, using GPM6A antibody (STJ111550) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-GPM6A antibody (1-100) (STJ111550)

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STJ111550

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GPM6A (1-100) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GPM6A
Gene ID: 2823
Uniprot ID: GPM6A_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GPM6A (NP_005268.1).
Immunogen Sequence: MEENMEEGQTQKGCFECCIK CLGGIPYASLIATILLYAGV ALFCGCGHEALSGTVNILQT YFEMARTAGDTLDVFTMIDI FKYVIYGIAAAFFVYGILLM
Post Translational Modifications N-glycosylated. Palmitoylated by ZDHHC17/HIP14.
Function Involved in neuronal differentiation, including differentiation and migration of neuronal stem cells. Plays a role in neuronal plasticity and is involved in neurite and filopodia outgrowth, filopodia motility and probably synapse formation. GPM6A-induced filopodia formation involves mitogen-activated protein kinase (MAPK) and Src signaling pathways. May be involved in neuronal NGF-dependent Ca(2+) influx. May be involved in regulation of endocytosis and intracellular trafficking of G-protein-coupled receptors (GPCRs).enhances internalization and recycling of mu-type opioid receptor.
Protein Name Neuronal Membrane Glycoprotein M6-A
M6a
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Cell Projection
Axon
Growth Cone
Dendritic Spine
Filopodium
Neuron Projection
Localizes To Cholesterol-Rich Lipid Rafts Of The Plasma Membrane Of Hippocampal Neurons
Localized To Plasma Membrane Of Cell Bodies And Neurites Of Hippocampal Neurons
Localized In Membrane Protrusions (Filopodia And Spines) Of Primary Hippocampal Neurons
Localized To The Growth Cone Edge Membrane Of Elongating Axons
Alternative Antibody Names Anti-Neuronal Membrane Glycoprotein M6-A antibody
Anti-M6a antibody
Anti-GPM6A antibody
Anti-M6A antibody

Information sourced from Uniprot.org

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