Anti-GPHN antibody (520-769) (STJ111307)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal GPHN (520-769) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GPHN |
| Gene ID | 10243 |
| Uniprot ID | GEPH_HUMAN |
| Immunogen Region | 520-769 |
| Immunogen Sequence | TVGVTEVEVNKFPVVAVMST GNELLNPEDDLLPGKIRDSN RSTLLATIQEHGYPTINLGI VGDNPDDLLNALNEGISRAD VIITSGGVSMGEKDYLKQVL DIDLHAQIHFGRVFMKPGLP TTFATLDIDGVRKIIFALPG NPVSAVVTCNLFVVPALRKM QGILDPRPTIIKARLSCDVK LDPRPEYHRCILTWHHQEPL PWAQSTGNQMSSRLMSMRSA NGLLMLPPKTEQYVELHKG |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 520-769 of human Gephyrin (NP_065857.1). |
Additional Info
| Post Translational Modifications | Palmitoylated. Palmitoylation is stimulated by GABA type A receptors activity. Palmitoylation by ZDHHC12 regulates clustering at synapses. |
| Function | Microtubule-associated protein involved in membrane protein-cytoskeleton interactions. It is thought to anchor the inhibitory glycine receptor (GLYR) to subsynaptic microtubules. Acts as a major instructive molecule at inhibitory synapses, where it also clusters GABA type A receptors. Also has a catalytic activity and catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated. Subsequently, molybdate is inserted into adenylated molybdopterin and AMP is released. |
| Protein Name | Gephyrin Includes - Molybdopterin AdenylyltransferaseMpt AdenylyltransferaseDomain G - Molybdopterin MolybdenumtransferaseMpt Mo-TransferaseDomain E |
| Database Links | Reactome: R-HSA-947581 |
| Cellular Localisation | Postsynaptic Cell MembraneLipid-AnchorCytoplasmic SideCell MembraneCytoplasmCytosolCytoskeletonCell ProjectionDendritePostsynaptic DensityCytoplasmic Face Of Glycinergic Postsynaptic MembranesForms Clusters At Synapses |
| Alternative Antibody Names | Anti-Gephyrin Includes - Molybdopterin Adenylyltransferase antibodyAnti-Mpt Adenylyltransferase antibodyAnti-Domain G - Molybdopterin Molybdenumtransferase antibodyAnti-Mpt Mo-Transferase antibodyAnti-Domain E antibodyAnti-GPHN antibodyAnti-GPH antibodyAnti-KIAA1385 antibody |
Information sourced from Uniprot.org