• Immunofluorescence analysis of paraffin-embedded mouse colon using GPA33 Rabbit polyclonal antibody (STJ117321) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using GPA33 Rabbit polyclonal antibody (STJ117321) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-GPA33 antibody (22-235) (STJ117321)

SKU:
STJ117321

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GPA33 (22-235) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GPA33
Gene ID: 10223
Uniprot ID: GPA33_HUMAN
Immunogen Region: 22-235
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 22-235 of human GPA33 (NP_005805.1).
Immunogen Sequence: ISVETPQDVLRASQGKSVTL PCTYHTSTSSREGLIQWDKL LLTHTERVVIWPFSNKNYIH GELYKNRVSISNNAEQSDAS ITIDQLTMADNGTYECSVSL MSDLEGNTKSRVRLLVLVPP SKPECGIEGETIIGNNIQLT CQSKEGSPTPQYSWKRYNIL NQEQPLAQPASGQPVSLKNI STDTSGYYICTSSNEEGTQF CNITVAVRSPSMNV
Tissue Specificity Expressed in normal gastrointestinal epithelium and in 95% of colon cancers.
Post Translational Modifications N-glycosylated, contains approximately 8 kDa of N-linked carbohydrate. Palmitoylated.
Function May play a role in cell-cell recognition and signaling.
Protein Name Cell Surface A33 Antigen
Glycoprotein A33
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Cell Surface A33 Antigen antibody
Anti-Glycoprotein A33 antibody
Anti-GPA33 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance