| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human/Mouse/Rat/Dog/Pig/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-GP91-PHOX/NOX2 (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:8000. WB: Approx 75kDa band observed in lysates of cell line Daudi and in Mouse Spleen lysates (calculated MW of 65.3kDa according to Human NP_000388.2 and Mouse NP_031833.3). This band was succe |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | CYBB |
| Gene ID: | 1536 |
| Uniprot ID: | CY24B_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_000388.2 |
| Immunogen Sequence: | CDKQEFQDAWKLPK |
| Post Translational Modifications | Glycosylated. Phosphorylated on Ser and Thr residues. Undergoes 'Lys-48'-linked polyubiquitination, likely by RNF145, triggering endoplasmic reticulum-associated degradation. |
| Function | Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc. |
| Protein Name | Cytochrome B-245 Heavy Chain Cgd91-Phox Cytochrome B(558 Subunit Beta Cytochrome B558 Subunit Beta Heme-Binding Membrane Glycoprotein Gp91phox Nadph Oxidase 2 Neutrophil Cytochrome B 91 Kda Polypeptide Superoxide-Generating Nadph Oxidase Heavy Chain Subunit Gp91-1 Gp91-Phox P22 Phagocyte B-Cytochrome |
| Database Links | Reactome: R-HSA-1222556 Reactome: R-HSA-1236973 Reactome: R-HSA-3299685 Reactome: R-HSA-4420097 Reactome: R-HSA-5668599 Reactome: R-HSA-6798695 Reactome: R-HSA-9013149 Reactome: R-HSA-9013404 Reactome: R-HSA-9013423 |
| Cellular Localisation | Cell Membrane Multi-Pass Membrane Protein As Unassembled Monomer May Localize To The Endoplasmic Reticulum |
| Alternative Antibody Names | Anti-Cytochrome B-245 Heavy Chain antibody Anti-Cgd91-Phox antibody Anti-Cytochrome B(558 Subunit Beta antibody Anti-Cytochrome B558 Subunit Beta antibody Anti-Heme-Binding Membrane Glycoprotein Gp91phox antibody Anti-Nadph Oxidase 2 antibody Anti-Neutrophil Cytochrome B 91 Kda Polypeptide antibody Anti-Superoxide-Generating Nadph Oxidase Heavy Chain Subunit antibody Anti-Gp91-1 antibody Anti-Gp91-Phox antibody Anti-P22 Phagocyte B-Cytochrome antibody Anti-CYBB antibody Anti-NOX2 antibody |
Information sourced from Uniprot.org