• Western blot analysis of extracts of various cell lines, using GOT2 antibody (STJ28995) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Immunohistochemistry analysis of paraffin-embedded human liver using GOT2 rabbit polyclonal antibody (STJ28995) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of C6 cells using GOT2 antibody (STJ28995) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of BALB-3T3 cells using GOT2 antibody (STJ28995) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-GOT2 antibody (30-200) (STJ28995)

SKU:
STJ28995

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GOT2 (30-200) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GOT2
Gene ID: 2806
Uniprot ID: AATM_HUMAN
Immunogen Region: 30-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 30-200 of human GOT2 (NP_002071.2).
Immunogen Sequence: SSWWTHVEMGPPDPILGVTE AFKRDTNSKKMNLGVGAYRD DNGKPYVLPSVRKAEAQIAA KNLDKEYLPIGGLAEFCKAS AELALGENSEVLKSGRFVTV QTISGTGALRIGASFLQRFF KFSRDVFLPKPTWGNHTPIF RDAGMQLQGYRYYDPKTCGF DFTGAVEDISK
Function Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). As a member of the malate-aspartate shuttle, it has a key role in the intracellular NAD(H) redox balance. Is important for metabolite exchange between mitochondria and cytosol, and for amino acid metabolism. Facilitates cellular uptake of long-chain free fatty acids.
Protein Name Aspartate Aminotransferase - Mitochondrial
Maspat
Fatty Acid-Binding Protein
Fabp-1
Glutamate Oxaloacetate Transaminase 2
Kynurenine Aminotransferase 4
Kynurenine Aminotransferase Iv
Kynurenine--Oxoglutarate Transaminase 4
Kynurenine--Oxoglutarate Transaminase Iv
Plasma Membrane-Associated Fatty Acid-Binding Protein
Fabppm
Transaminase A
Database Links Reactome: R-HSA-1614558
Reactome: R-HSA-389661
Reactome: R-HSA-70263
Reactome: R-HSA-8963693
Reactome: R-HSA-8964539
Cellular Localisation Mitochondrion Matrix
Cell Membrane
Exposure To Alcohol Promotes Translocation To The Cell Membrane
Alternative Antibody Names Anti-Aspartate Aminotransferase - Mitochondrial antibody
Anti-Maspat antibody
Anti-Fatty Acid-Binding Protein antibody
Anti-Fabp-1 antibody
Anti-Glutamate Oxaloacetate Transaminase 2 antibody
Anti-Kynurenine Aminotransferase 4 antibody
Anti-Kynurenine Aminotransferase Iv antibody
Anti-Kynurenine--Oxoglutarate Transaminase 4 antibody
Anti-Kynurenine--Oxoglutarate Transaminase Iv antibody
Anti-Plasma Membrane-Associated Fatty Acid-Binding Protein antibody
Anti-Fabppm antibody
Anti-Transaminase A antibody
Anti-GOT2 antibody
Anti-KYAT4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance