• Immunofluorescence analysis of U2OS cells using GOT1 antibody (STJ28385). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human colon using GOT1 antibody (STJ28385) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human lung cancer using GOT1 antibody (STJ28385) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat heart using GOT1 antibody (STJ28385) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using GOT1 antibody (STJ28385) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-GOT1 antibody (244-413) (STJ28385)

SKU:
STJ28385

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GOT1 (244-413) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:10-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GOT1
Gene ID: 2805
Uniprot ID: AATC_HUMAN
Immunogen Region: 244-413
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 244-413 of human GOT1 (NP_002070.1).
Immunogen Sequence: FVSEGFEFFCAQSFSKNFGL YNERVGNLTVVGKEPESILQ VLSQMEKIVRITWSNPPAQG ARIVASTLSNPELFEEWTGN VKTMADRILTMRSELRARLE ALKTPGTWNHITDQIGMFSF TGLNPKQVEYLVNEKHIYLL PSGRINVSGLTTKNLDYVAT SIHEAVTKIQ
Function Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain. In addition, catalyzes (2S)-2-aminobutanoate, a by-product in the cysteine biosynthesis pathway.
Protein Name Aspartate Aminotransferase - Cytoplasmic
Caspat
Cysteine Aminotransferase - Cytoplasmic
Cysteine Transaminase - Cytoplasmic
Ccat
Glutamate Oxaloacetate Transaminase 1
Transaminase A
Database Links Reactome: R-HSA-1237112
Reactome: R-HSA-70263
Reactome: R-HSA-8963693
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Aspartate Aminotransferase - Cytoplasmic antibody
Anti-Caspat antibody
Anti-Cysteine Aminotransferase - Cytoplasmic antibody
Anti-Cysteine Transaminase - Cytoplasmic antibody
Anti-Ccat antibody
Anti-Glutamate Oxaloacetate Transaminase 1 antibody
Anti-Transaminase A antibody
Anti-GOT1 antibody

Information sourced from Uniprot.org

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