• Western blot analysis of extracts of various cell lines, using PIST/PIST/GOPC antibody (STJ29649) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-GOPC antibody (195-454) (STJ29649)

SKU:
STJ29649

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GOPC (195-454) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GOPC
Gene ID: 57120
Uniprot ID: GOPC_HUMAN
Immunogen Region: 195-454
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 195-454 of human PIST/PIST/GOPC (NP_001017408.1).
Immunogen Sequence: ARLAAKYLDKELAGRVQQIQ LLGRDMKGPAHDKLWNQLEA EIHLHRHKTVIRACRGRNDL KRPMQAPPGHDQDSLKKSQG VGPIRKVLLLKEDHEGLGIS ITGGKEHGVPILISEIHPGQ PADRCGGLHVGDAILAVNGV NLRDTKHKEAVTILSQQRGE IEFEVVYVAPEVDSDDENVE YEDESGHRYRLYLDELEGGG NPGASCKDTSGEIKVLQGFN KKAVTDTHENGDLGTASET
Tissue Specificity Ubiquitously expressed.
Function Plays a role in intracellular protein trafficking and degradation. May regulate CFTR chloride currents and acid-induced ASIC3 currents by modulating cell surface expression of both channels. May also regulate the intracellular trafficking of the ADR1B receptor. May play a role in autophagy. Together with MARCHF2 mediates the ubiquitination and lysosomal degradation of CFTR. Overexpression results in CFTR intracellular retention and lysosomaldegradation in the lysosomes.
Protein Name Golgi-Associated Pdz And Coiled-Coil Motif-Containing Protein
Cftr-Associated Ligand
Fused In Glioblastoma
Pdz Protein Interacting Specifically With Tc10
Pist
Database Links Reactome: R-HSA-5627083
Reactome: R-HSA-9013406
Cellular Localisation Cytoplasm
Golgi Apparatus Membrane
Peripheral Membrane Protein
Golgi Apparatus
Trans-Golgi Network Membrane
Synapse
Postsynaptic Density
Cell Projection
Dendrite
Enriched In Synaptosomal And Postsynaptic Densities (Psd) Fractions
Expressed In Cell Bodies And Dendrites Of Purkinje Cells
Localized At The Trans-Golgi Network (Tgn) Of Spermatids And The Medulla Of Round Spermatides
Alternative Antibody Names Anti-Golgi-Associated Pdz And Coiled-Coil Motif-Containing Protein antibody
Anti-Cftr-Associated Ligand antibody
Anti-Fused In Glioblastoma antibody
Anti-Pdz Protein Interacting Specifically With Tc10 antibody
Anti-Pist antibody
Anti-GOPC antibody
Anti-CAL antibody
Anti-FIG antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance