• Immunofluorescence analysis of HepG2 cells using GOLGA1 Rabbit polyclonal antibody (STJ116890) at dilution of 1:150. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using GOLGA1 Rabbit polyclonal antibody (STJ116890) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-GOLGA1 antibody (1-300) (STJ116890)

SKU:
STJ116890

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GOLGA1 (1-300) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GOLGA1
Gene ID: 2800
Uniprot ID: GOGA1_HUMAN
Immunogen Region: 1-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GOLGA1 (NP_002068.1).
Immunogen Sequence: MFAKLKKKIAEETAVAQRPG GATRIPRSVSKESVASMGAD SGDDFASDGSSSREDLSSQL LRRNEQIRKLEARLSDYAEQ VRNLQKIKEKLEIALEKHQD SSMRKFQEQNETFQANRAKM AEGLALALARKDQEWSEKMD QLEKEKNILTAQLQEMKNQS MNLFQRRDEMDELEGFQQQE LSKIKHMLLKKEESLGKMEQ ELEARTRELSRTQEELMNSN QMSSDLSQKLEELQRHYST
Function Involved in vesicular trafficking at the Golgi apparatus level. Involved in endosome-to-Golgi trafficking.
Protein Name Golgin Subfamily A Member 1
Golgin-97
Database Links Reactome: R-HSA-6811440
Cellular Localisation Golgi Apparatus Membrane
Peripheral Membrane Protein
Golgi Apparatus
Trans-Golgi Network Membrane
Cytoplasmic Vesicle
Secretory Vesicle
Acrosome
Alternative Antibody Names Anti-Golgin Subfamily A Member 1 antibody
Anti-Golgin-97 antibody
Anti-GOLGA1 antibody

Information sourced from Uniprot.org

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