• Western blot analysis of extracts of mouse lung, using GNRH1 antibody (STJ27592) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of Rat kidney, using GNRH1 antibody (STJ27592) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
  • Immunofluorescence analysis of SH-SY5Y cells using GNRH1 rabbit polyclonal antibody (STJ27592) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using GNRH1 rabbit polyclonal antibody (STJ27592) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-GNRH1 antibody (24-92) (STJ27592)

SKU:
STJ27592

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GNRH1 (24-92) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GNRH1
Gene ID: 2796
Uniprot ID: GON1_HUMAN
Immunogen Region: 24-92
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 24-92 of human GNRH1 (NP_001076580.1).
Immunogen Sequence: QHWSYGLRPGGKRDAENLID SFQEIVKEVGQLAETQRFEC TTHQPRSPLRDLKGALESLI EEETGQKKI
Post Translational Modifications Gonadoliberin-1: The precursor is cleaved by ACE, which removes the Gly-Lys-Arg peptide at the C-terminus, leading to mature hormone. The mature form of Gonadoliberin-1 is also cleaved and degraded by ACE.
Function Stimulates the secretion of gonadotropins.it stimulates the secretion of both luteinizing and follicle-stimulating hormones.
Protein Name Progonadoliberin-1
Progonadoliberin I Cleaved Into - Gonadoliberin-1
Gonadoliberin I
Gonadorelin
Gonadotropin-Releasing Hormone I
Gnrh-I
Luliberin I
Luteinizing Hormone-Releasing Hormone I
Lh-Rh I - Gnrh-Associated Peptide 1
Gnrh-Associated Peptide I
Database Links Reactome: R-HSA-375281
Reactome: R-HSA-416476
Cellular Localisation Secreted
Alternative Antibody Names Anti-Progonadoliberin-1 antibody
Anti-Progonadoliberin I Cleaved Into - Gonadoliberin-1 antibody
Anti-Gonadoliberin I antibody
Anti-Gonadorelin antibody
Anti-Gonadotropin-Releasing Hormone I antibody
Anti-Gnrh-I antibody
Anti-Luliberin I antibody
Anti-Luteinizing Hormone-Releasing Hormone I antibody
Anti-Lh-Rh I - Gnrh-Associated Peptide 1 antibody
Anti-Gnrh-Associated Peptide I antibody
Anti-GNRH1 antibody
Anti-GNRH antibody
Anti-GRH antibody
Anti-LHRH antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance