• Immunoprecipitation analysis of 200 Mu g extracts of 293T cells using 3 Mu g GNL3 antibody (STJ28542). Western blot was performed from the immunoprecipitate using GNL3 antibody (STJ28542) at a dilution of 1:500.
  • Immunofluorescence analysis of MCF7 cells using GNL3 Rabbit polyclonal antibody (STJ28542). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from 293T cells, using GNL3 Rabbit polyclonal antibody (STJ28542) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-GNL3 antibody (1-240) (STJ28542)

SKU:
STJ28542

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GNL3 (1-240) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:10-1:100
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GNL3
Gene ID: 26354
Uniprot ID: GNL3_HUMAN
Immunogen Region: 1-240
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human GNL3 (NP_996562.1).
Immunogen Sequence: MTCHKRYKIQKKVREHHRKL RKEAKKRGHKKPRKDPGVPN SAPFKEALLREAELRKQRLE ELKQQQKLDRQKELEKKRKL ETNPDIKPSNVEPMEKEFGL CKTENKAKSGKQNSKKLYCQ ELKKVIEASDVVLEVLDARD PLGCRCPQVEEAIVQSGQKK LVLILNKSDLVPKENLESWL NYLKKELPTVVFRASTKPKD KGKITKRVKAKKNAAPFRSE VCFGKEGLWKLLGGFQETC
Tissue Specificity Increased levels in lung tissue in cancer patients.
Function May be required to maintain the proliferative capacity of stem cells. Stabilizes MDM2 by preventing its ubiquitination, and hence proteasomal degradation.
Protein Name Guanine Nucleotide-Binding Protein-Like 3
E2-Induced Gene 3 Protein
Novel Nucleolar Protein 47
Nnp47
Nucleolar Gtp-Binding Protein 3
Nucleostemin
Database Links Reactome: R-HSA-6791226
Cellular Localisation Nucleus
Nucleolus
Shuttles Between The Nucleus And Nucleolus
Alternative Antibody Names Anti-Guanine Nucleotide-Binding Protein-Like 3 antibody
Anti-E2-Induced Gene 3 Protein antibody
Anti-Novel Nucleolar Protein 47 antibody
Anti-Nnp47 antibody
Anti-Nucleolar Gtp-Binding Protein 3 antibody
Anti-Nucleostemin antibody
Anti-GNL3 antibody
Anti-E2IG3 antibody
Anti-NS antibody

Information sourced from Uniprot.org

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