Anti-GNE antibody (592-641 aa) (STJ93278)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human GNE at the amino acid range 592-641
General Information
| Short Description | Rabbit polyclonal anti-Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase (592-641 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:5000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GNE |
| Gene ID | 10020 |
| Uniprot ID | GLCNE_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human GNE at the amino acid range 592-641 |
| Immunogen Region | 592-641 aa |
| Specificity | GLCNE Polyclonal Antibody detects endogenous levels of GLCNE protein. |
Additional Info
| Post Translational Modifications | Phosphorylated. Phosphorylation by PKC activates the UDP-N-acetylglucosamine 2-epimerase activity. |
| Function | Bifunctional enzyme that possesses both UDP-N-acetylglucosamine 2-epimerase and N-acetylmannosamine kinase activities, and serves as the initiator of the biosynthetic pathway leading to the production of N-acetylneuraminic acid (NeuAc), a critical precursor in the synthesis of sialic acids. By catalyzing this pivotal and rate-limiting step in sialic acid biosynthesis, this enzyme assumes a pivotal role in governing the regulation of cell surface sialylation, playing a role in embryonic angiogenesis. Sialic acids represent a category of negatively charged sugars that reside on the surface of cells as terminal components of glycoconjugates and mediate important functions in various cellular processes, including cell adhesion, signal transduction, and cellular recognition. |
| Protein Name | Bifunctional Udp-N-Acetylglucosamine 2-Epimerase/N-Acetylmannosamine KinaseUdp-Glcnac-2-Epimerase/Manac Kinase Includes - Udp-N-Acetylglucosamine 2-EpimeraseHydrolyzingUdp-Glcnac-2-EpimeraseUridine Diphosphate-N-Acetylglucosamine-2-Epimerase - N-Acetylmannosamine KinaseManac Kinase |
| Database Links | Reactome: R-HSA-4085001Reactome: R-HSA-4085011 |
| Cellular Localisation | CytoplasmCytosol |
| Alternative Antibody Names | Anti-Bifunctional Udp-N-Acetylglucosamine 2-Epimerase/N-Acetylmannosamine Kinase antibodyAnti-Udp-Glcnac-2-Epimerase/Manac Kinase Includes - Udp-N-Acetylglucosamine 2-Epimerase antibodyAnti-Hydrolyzing antibodyAnti-Udp-Glcnac-2-Epimerase antibodyAnti-Uridine Diphosphate-N-Acetylglucosamine-2-Epimerase - N-Acetylmannosamine Kinase antibodyAnti-Manac Kinase antibodyAnti-GNE antibodyAnti-GLCNE antibodyAnti-IBM2 antibody |
Information sourced from Uniprot.org