Anti-GNAT3 antibody (Internal Region) (STJ13101142)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from the internal region of mouse GNAT3 conjugated to blue carrier protein was used as the antigen. The antigen shares 87% identity with the rat sequence.
General Information
| Short Description | Rabbit polyclonal anti-GNAT3 (Internal Region) for use in IHC and WB in Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC, WB. A dilution of 1:1000 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | Gnat3 |
| Gene ID | 242851 |
| Uniprot ID | GNAT3_MOUSE |
| Immunogen | A synthetic peptide from the internal region of mouse GNAT3 conjugated to blue carrier protein was used as the antigen. The antigen shares 87% identity with the rat sequence. |
| Immunogen Region | Internal Region |
| Specificity | Specific for GNAT3. |
Additional Info
| Post Translational Modifications | Potential N-myristoylation may anchor alpha-subunit to the inner surface of plasma membrane. |
| Function | Guanine nucleotide-binding protein (G protein) alpha subunit playing a prominent role in bitter and sweet taste transduction as well as in umami (monosodium glutamate, monopotassium glutamate, and inosine monophosphate) taste transduction. Transduction by this alpha subunit involves coupling of specific cell-surface receptors with a cGMP-phosphodiesterase.Activation of phosphodiesterase lowers intracellular levels of cAMP and cGMP which may open a cyclic nucleotide-suppressible cation channel leading to influx of calcium, ultimately leading to release of neurotransmitter. Indeed, denatonium and strychnine induce transient reduction in cAMP and cGMP in taste tissue, whereas this decrease is inhibited by GNAT3 antibody. Gustducin heterotrimer transduces response to bitter and sweet compounds via regulation of phosphodiesterase for alpha subunit, as well as via activation of phospholipase C for beta and gamma subunits, with ultimate increase inositol trisphosphate and increase of intracellular Calcium. GNAT3 can functionally couple to taste receptors to transmit intracellular signal: receptor heterodimer TAS1R2/TAS1R3 senses sweetness and TAS1R1/TAS1R3 transduces umami taste, whereas the T2R family GPCRs act as bitter sensors. Also functions as lumenal sugar sensors in the gut to control the expression of the Na+-glucose transporter SGLT1 in response to dietaty sugar, as well as the secretion of Glucagon-like peptide-1, GLP-1 and glucose-dependent insulinotropic polypeptide, GIP. Thus, may modulate the gut capacity to absorb sugars, with implications in malabsorption syndromes and diet-related disorders including diabetes and obesity. |
| Protein Name | Guanine Nucleotide-Binding Protein G(T Subunit Alpha-3Gustducin Alpha-3 Chain |
| Database Links | Reactome: R-MMU-170670Reactome: -MMU-381771Reactome: -MMU-392170Reactome: -MMU-418594Reactome: -MMU-9009391Reactome: -MMU-9717207 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Guanine Nucleotide-Binding Protein G(T Subunit Alpha-3 antibodyAnti-Gustducin Alpha-3 Chain antibodyAnti-Gnat3 antibody |
Information sourced from Uniprot.org