• Immunofluorescence analysis of NIH-3T3 cells using GNA13 Rabbit polyclonal antibody (STJ11104291) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using GNA13 Rabbit polyclonal antibody (STJ11104291) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of GNA13 in paraffin-embedded mouse kidney using GNA13 Rabbit polyclonal antibody (STJ11104291) (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of GNA13 in paraffin-embedded human stomach using GNA13 Rabbit polyclonal antibody (STJ11104291) (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using GNA13 Rabbit polyclonal antibody (STJ11104291) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-GNA13 antibody (1-210) (STJ11104291)

SKU:
STJ11104291

Current Stock:
Host: Rabbit
Applications: WB/IF/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Guanine Nucleotide-Binding Protein Subunit Alpha-13 (1-210) is suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GNA13
Gene ID: 10672
Uniprot ID: GNA13_HUMAN
Immunogen Region: 1-210
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human GNA13 (NP_006563.2).
Immunogen Sequence: MADFLPSRSVLSVCFPGCLL TSGEAEQQRKSKEIDKCLSR EKTYVKRLVKILLLGAGESG KSTFLKQMRIIHGQDFDQRA REEFRPTIYSNVIKGMRVLV DAREKLHIPWGDNSNQQHGD KMMSFDTRAPMAAQGMVETR VFLQYLPAIRALWADSGIQN AYDRRREFQLGESVKYFLDN LDKLGEPDYIPSQQDILLAR RPTKGIHEYD
Tissue Specificity Expressed in testis, including in Leydig cells and in the seminiferous epithelium, in differentiating cells from the spermatogonia to mature spermatozoa stages and round spermatids (at protein level). Expressed in 99.2% of spermatozoa from healthy individuals, but only in 28.6% of macrocephalic spermatozoa from infertile patients (at protein level).
Post Translational Modifications Palmitoylation is critical for proper membrane localization and signaling. Phosphorylation on Thr-203 by PKA destabilizes the heterotrimer of alpha, beta and gamma, and inhibits Rho activation.
Function Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Activates effector molecule RhoA by binding and activating RhoGEFs (ARHGEF1/p115RhoGEF, ARHGEF11/PDZ-RhoGEF and ARHGEF12/LARG). GNA13-dependent Rho signaling subsequently regulates transcription factor AP-1 (activating protein-1). Promotes tumor cell invasion and metastasis by activating RhoA/ROCK signaling pathway. Inhibits CDH1-mediated cell adhesion in process independent from Rho activation.
Protein Name Guanine Nucleotide-Binding Protein Subunit Alpha-13
G Alpha-13
G-Protein Subunit Alpha-13
Database Links Reactome: R-HSA-193648
Reactome: R-HSA-416482
Reactome: R-HSA-428930
Reactome: R-HSA-456926
Reactome: R-HSA-9013148
Reactome: R-HSA-9013149
Cellular Localisation Cell Membrane
Lipid-Anchor
Melanosome
Cytoplasm
Nucleus
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Detected In The Cytoplasm Of Leydig Cells And In The Seminiferous Epithelium
Including Differentiating Cells From The Spermatogonia To Mature Spermatozoa Stages
In Round Spermatids
Also Present In The Nuclei
Alternative Antibody Names Anti-Guanine Nucleotide-Binding Protein Subunit Alpha-13 antibody
Anti-G Alpha-13 antibody
Anti-G-Protein Subunit Alpha-13 antibody
Anti-GNA13 antibody

Information sourced from Uniprot.org

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