• Western blot analysis of extracts of various cell lines, using Gm13125 antibody (STJ110394) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of paraffin-embedded mouse ovary using Gm13125 Rabbit polyclonal antibody (STJ110394) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse ovary using Gm13125 rabbit polyclonal antibody (STJ110394) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded rat ovary using Gm13125 Rabbit polyclonal antibody (STJ110394) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat ovary using Gm13125 rabbit polyclonal antibody (STJ110394) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using Gm13125 antibody (STJ110394) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Gm13125 antibody (67-274) (STJ110394)

SKU:
STJ110394

€140.63 - €653.13
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Gm13125 (67-274) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRAMEF20
Gene ID: 645425
Uniprot ID: PRA20_HUMAN
Immunogen Region: 67-274
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 67-274 of mouse Gm13125 (NP_001108549.1).
Immunogen Sequence: GALKKRTPYLETQLQVVLEE VDKLLIQEVHPREYKLEVLD LRSVGQNYLNVWPGAMDDWL PKTTQTDPCCSKTVMTQPLK VLVDLQLNNRGQSRLFSYLV QWSDRRKGLLQLYCNKLQIW SPSQQNYRKLSQKVNLDYVE TLGLHSSCSPSFLLNFAPYL RQMRNLRKLALSNIREEHII SPEKRRCIITRFTLQILKLE SLQKLHLD
Protein Name Prame Family Member 20
Alternative Antibody Names Anti-Prame Family Member 20 antibody
Anti-PRAMEF20 antibody
Anti-PRAMEF21 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance